Fabrication and characterization of porous silicon nanoparticles for siRNA delivery

Phikunthiong Kopermsub; Varissaporn Mayen; Steven J P Mcinnes; Nicolas H. Voelcker

doi:10.1109/NANO.2011.6144461

Fabrication and characterization of porous silicon nanoparticles for siRNA delivery

Phikunthiong Kopermsub, Varissaporn Mayen, Steven J P Mcinnes, Nicolas H. Voelcker

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Other › peer-review

1 Citation (Scopus)

Abstract

RNA interference (RNAi) using small interfering RNA (siRNA) has been proposed for the therapy of human diseases and in particular cancers. Due to the instability and physicochemical properties of siRNA, the development of siRNA delivery system has attracted much attention. This study aimed at the fabrication of porous silicon nanoparticles (pSi NPs) as carriers for siRNA. pSi NPs possess a high surface area and are biodegradable and biocompatible. pSi NPs with two types of surface chemistry (oxidized and amine-functionalized) were fabricated and characterized. The surface area of pSi NPs was found to be 390 m2/g with a pore width of 9.2 nm. To investigate siRNA loading efficiency of pSi NPs, duplex siRNA was incubated with nanoparticles for 1 hour. The loading capacity of oxidized pSi NPs and amine-functionalized pSi NPs was found to be 1.95 g/mg particles and 2.02 g/mg particles, respectively. Further investigation on cellular uptake and gene silencing of siRNA-loaded pSi nanoparticles will be conducted to provide more details on their possible use for siRNA delivery.

Original language	English
Title of host publication	2011 11th IEEE International Conference on Nanotechnology, NANO 2011
Pages	830-832
Number of pages	3
DOIs	https://doi.org/10.1109/NANO.2011.6144461
Publication status	Published - 2011
Externally published	Yes
Event	IEEE International Conference on Nanotechnology 2011 - Portland, United States of America Duration: 15 Aug 2011 → 19 Aug 2011 Conference number: 11th https://ieeexplore.ieee.org/xpl/conhome/6125891/proceeding (Proceedings)

Conference

Conference	IEEE International Conference on Nanotechnology 2011
Abbreviated title	IEEE-NANO 2011
Country/Territory	United States of America
City	Portland
Period	15/08/11 → 19/08/11
Internet address	https://ieeexplore.ieee.org/xpl/conhome/6125891/proceeding (Proceedings)

Keywords

amine-functionalization
porous silicon nanoparticles
siRNA delivery

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/NANO.2011.6144461

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title = "Fabrication and characterization of porous silicon nanoparticles for siRNA delivery",

abstract = "RNA interference (RNAi) using small interfering RNA (siRNA) has been proposed for the therapy of human diseases and in particular cancers. Due to the instability and physicochemical properties of siRNA, the development of siRNA delivery system has attracted much attention. This study aimed at the fabrication of porous silicon nanoparticles (pSi NPs) as carriers for siRNA. pSi NPs possess a high surface area and are biodegradable and biocompatible. pSi NPs with two types of surface chemistry (oxidized and amine-functionalized) were fabricated and characterized. The surface area of pSi NPs was found to be 390 m2/g with a pore width of 9.2 nm. To investigate siRNA loading efficiency of pSi NPs, duplex siRNA was incubated with nanoparticles for 1 hour. The loading capacity of oxidized pSi NPs and amine-functionalized pSi NPs was found to be 1.95 g/mg particles and 2.02 g/mg particles, respectively. Further investigation on cellular uptake and gene silencing of siRNA-loaded pSi nanoparticles will be conducted to provide more details on their possible use for siRNA delivery.",

keywords = "amine-functionalization, porous silicon nanoparticles, siRNA delivery",

author = "Phikunthiong Kopermsub and Varissaporn Mayen and Mcinnes, {Steven J P} and Voelcker, {Nicolas H.}",

year = "2011",

doi = "10.1109/NANO.2011.6144461",

language = "English",

isbn = "9781457715143",

pages = "830--832",

booktitle = "2011 11th IEEE International Conference on Nanotechnology, NANO 2011",

note = "IEEE International Conference on Nanotechnology 2011, IEEE-NANO 2011 ; Conference date: 15-08-2011 Through 19-08-2011",

url = "https://ieeexplore.ieee.org/xpl/conhome/6125891/proceeding",

}

Kopermsub, P, Mayen, V, Mcinnes, SJP & Voelcker, NH 2011, Fabrication and characterization of porous silicon nanoparticles for siRNA delivery. in 2011 11th IEEE International Conference on Nanotechnology, NANO 2011., 6144461, pp. 830-832, IEEE International Conference on Nanotechnology 2011, Portland, Oregon, United States of America, 15/08/11. https://doi.org/10.1109/NANO.2011.6144461

TY - GEN

T1 - Fabrication and characterization of porous silicon nanoparticles for siRNA delivery

AU - Kopermsub, Phikunthiong

AU - Mayen, Varissaporn

AU - Mcinnes, Steven J P

AU - Voelcker, Nicolas H.

N1 - Conference code: 11th

PY - 2011

Y1 - 2011

N2 - RNA interference (RNAi) using small interfering RNA (siRNA) has been proposed for the therapy of human diseases and in particular cancers. Due to the instability and physicochemical properties of siRNA, the development of siRNA delivery system has attracted much attention. This study aimed at the fabrication of porous silicon nanoparticles (pSi NPs) as carriers for siRNA. pSi NPs possess a high surface area and are biodegradable and biocompatible. pSi NPs with two types of surface chemistry (oxidized and amine-functionalized) were fabricated and characterized. The surface area of pSi NPs was found to be 390 m2/g with a pore width of 9.2 nm. To investigate siRNA loading efficiency of pSi NPs, duplex siRNA was incubated with nanoparticles for 1 hour. The loading capacity of oxidized pSi NPs and amine-functionalized pSi NPs was found to be 1.95 g/mg particles and 2.02 g/mg particles, respectively. Further investigation on cellular uptake and gene silencing of siRNA-loaded pSi nanoparticles will be conducted to provide more details on their possible use for siRNA delivery.

AB - RNA interference (RNAi) using small interfering RNA (siRNA) has been proposed for the therapy of human diseases and in particular cancers. Due to the instability and physicochemical properties of siRNA, the development of siRNA delivery system has attracted much attention. This study aimed at the fabrication of porous silicon nanoparticles (pSi NPs) as carriers for siRNA. pSi NPs possess a high surface area and are biodegradable and biocompatible. pSi NPs with two types of surface chemistry (oxidized and amine-functionalized) were fabricated and characterized. The surface area of pSi NPs was found to be 390 m2/g with a pore width of 9.2 nm. To investigate siRNA loading efficiency of pSi NPs, duplex siRNA was incubated with nanoparticles for 1 hour. The loading capacity of oxidized pSi NPs and amine-functionalized pSi NPs was found to be 1.95 g/mg particles and 2.02 g/mg particles, respectively. Further investigation on cellular uptake and gene silencing of siRNA-loaded pSi nanoparticles will be conducted to provide more details on their possible use for siRNA delivery.

KW - amine-functionalization

KW - porous silicon nanoparticles

KW - siRNA delivery

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M3 - Conference Paper

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SN - 9781457715143

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BT - 2011 11th IEEE International Conference on Nanotechnology, NANO 2011

T2 - IEEE International Conference on Nanotechnology 2011

Y2 - 15 August 2011 through 19 August 2011

ER -

Fabrication and characterization of porous silicon nanoparticles for siRNA delivery

Abstract

Conference

Keywords

UN SDGs

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