Extracellular vesicle isolation and characterization: Toward clinical application

Rong Xu; David W. Greening; Hong Jian Zhu; Nobuhiro Takahashi; Richard J. Simpson

doi:10.1172/JCI81129

Extracellular vesicle isolation and characterization: Toward clinical application

Rong Xu, David W. Greening, Hong Jian Zhu, Nobuhiro Takahashi, Richard J. Simpson

Research output: Contribution to journal › Review Article › Other › peer-review

703 Citations (Scopus)

Abstract

Two broad categories of extracellular vesicles (EVs), exosomes and shed microvesicles (sMVs), which differ in size distribution as well as protein and RNA profiles, have been described. EVs are known to play key roles in cell-cell communication, acting proximally as well as systemically. This Review discusses the nature of EV subtypes, strategies for isolating EVs from both cell-culture media and body fluids, and procedures for quantifying EVs. We also discuss proteins selectively enriched in exosomes and sMVs that have the potential for use as markers to discriminate between EV subtypes, as well as various applications of EVs in clinical diagnosis.

Original language	English
Pages (from-to)	1152-1162
Number of pages	11
Journal	The Journal of Clinical Investigation
Volume	126
Issue number	4
DOIs	https://doi.org/10.1172/JCI81129
Publication status	Published - Apr 2016
Externally published	Yes

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10.1172/JCI81129Licence: CC BY

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title = "Extracellular vesicle isolation and characterization: Toward clinical application",

abstract = "Two broad categories of extracellular vesicles (EVs), exosomes and shed microvesicles (sMVs), which differ in size distribution as well as protein and RNA profiles, have been described. EVs are known to play key roles in cell-cell communication, acting proximally as well as systemically. This Review discusses the nature of EV subtypes, strategies for isolating EVs from both cell-culture media and body fluids, and procedures for quantifying EVs. We also discuss proteins selectively enriched in exosomes and sMVs that have the potential for use as markers to discriminate between EV subtypes, as well as various applications of EVs in clinical diagnosis.",

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AU - Zhu, Hong Jian

AU - Takahashi, Nobuhiro

AU - Simpson, Richard J.

PY - 2016/4

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AB - Two broad categories of extracellular vesicles (EVs), exosomes and shed microvesicles (sMVs), which differ in size distribution as well as protein and RNA profiles, have been described. EVs are known to play key roles in cell-cell communication, acting proximally as well as systemically. This Review discusses the nature of EV subtypes, strategies for isolating EVs from both cell-culture media and body fluids, and procedures for quantifying EVs. We also discuss proteins selectively enriched in exosomes and sMVs that have the potential for use as markers to discriminate between EV subtypes, as well as various applications of EVs in clinical diagnosis.

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Extracellular vesicle isolation and characterization: Toward clinical application

Abstract

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Extracellular vesicle isolation and characterization: Toward clinical application

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