Extra-ovarian production of mature viable mouse oocytes from frozen primary follicles

J. Carroll, D. G. Whittingham, M. J. Wood, E. Telfer, R. G. Gosden

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121 Citations (Scopus)

Abstract

Isolated primary mouse follicles can be frozen successfully and thawed in the presence of 1.5 M-DMSO. Similar proportions of freshly collected and frozen-thawed primary follicles undergo folliculogenesis in the absence of other ovarian tissue. Some of the mature oocytes recovered from these follicles were fertilized in vitro and, after transfer to pseudopregnant recipients at the 2-cell stage, developed into live young. Cryopreservation and extra-ovarian development of immature follicles provide a unique opportunity to store large numbers of female gametes.

Original languageEnglish
Pages (from-to)321-327
Number of pages7
JournalJournal of Reproduction and Fertility
Volume90
Issue number1
DOIs
Publication statusPublished - 1 Jan 1990
Externally publishedYes

Keywords

  • Cryopreservation
  • Embryo transfer
  • In-vitro fertilization
  • In-vitro folliculogenesis
  • Mouse
  • Oocyte
  • Oocyte maturation
  • Primary follicle

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