Extending the life span of acute neuronal tissue for imaging and electrophysiological studies

Yossi Buskila, Alba Bellot-Saez, Orsolya Kékesi, Morven Cameron, John Morley

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Slice preparations of neuronal tissue are among the most commonly used experimental approaches in the field of neuroscience. They are employed for a variety of techniques addressing questions across the entire neuroscience spectrum, including immunohistochemical, anatomical, and electrophysiological methods to study the properties of individual, and networks of neurons. In the past decades, slice preparations have provided information that has allowed us to develop our understanding of the central nervous system. Unlike cultures, slice preparations leave the topography of neurons and glia intact and therefore retain a considerable degree of functionality that allows molecular, cellular, and network investigations. However, a major limitation of using acute brain slices is their life span which is limited to 6–8 h due to intrinsic and extrinsic factors. Recently, new technological and methodological modifications have proved efficient in extending the life span of acute neuronal tissue. In this chapter, we will review the mechanisms leading to tissue deterioration and describe in detail the steps required to achieve a significant enhancement in neuronal viability and longevity.

Original languageEnglish
Title of host publicationBasic Neurobiology Techniques
EditorsNicholas J. D. Wright
Place of PublicationNew York, NY
PublisherHumana Press
Chapter10
Pages235-259
Number of pages25
Edition1st
ISBN (Electronic)9781493999446
ISBN (Print)9781493999439
DOIs
Publication statusPublished - 2020
Externally publishedYes

Publication series

NameNeuromethods
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • ATP
  • Bacteria
  • Brain slices
  • Braincubator
  • Electrophysiology
  • Incubation
  • Ischemia

Cite this

Buskila, Y., Bellot-Saez, A., Kékesi, O., Cameron, M., & Morley, J. (2020). Extending the life span of acute neuronal tissue for imaging and electrophysiological studies. In N. J. D. Wright (Ed.), Basic Neurobiology Techniques (1st ed., pp. 235-259). (Neuromethods). New York, NY: Humana Press. https://doi.org/10.1007/978-1-4939-9944-6_10