Enhanced consensus green protein variant 123 (eCGP123) is an extremely thermostable green fluorescent protein (GFP) that exhibits useful negative reversible photoswitching properties. eCGP123 was derived by the application of both a consensus engineering approach and a recursive evolutionary process. Diffraction-quality crystals of recombinant eCGP123 were obtained by the hanging-drop vapour-diffusion method using PEG 3350 as the precipitant. The eCGP123 crystal diffracted X-rays to 2.10 A Es resolution. The data were indexed in space group P1, with unit-cell parameters a = 74.63, b = 75.38, c = 84.51 A Es , a = 90.96, b = 89.92, y = 104.03o. The Matthews coefficient (VM = 2.26 A Es 3 Da-1) and a solvent content of 46 indicated that the asymmetric unit contained eight eCGP123 molecules.
|Pages (from-to)||1266 - 1268|
|Number of pages||3|
|Journal||Acta Crystallographica Section F: Structural Biology Communications|
|Publication status||Published - 2011|