TY - JOUR
T1 - Expression, purification, crystallization and preliminary crystallographic analysis of the PAB0955 gene product
AU - Gras, Stephanie
AU - Fernandez, Bernard
AU - Chaumont, Valerie
AU - Carpentier, Philippe
AU - Armengaud, Jean
AU - Housset, Dominique
PY - 2005
Y1 - 2005
N2 - PAB0955 from Pyrococcus abyssi is a prototype of a new Walker-type ATPase/GTPase conserved in archaea and eukaryota but not found in bacteria. PAB0955 has been expressed, purified and crystallized, and it has been shown that this thermostable protein is dimeric in reductive conditions. Crystals have been obtained either without nucleotide or in the presence of GDP or GTPgammaS. Preliminary X-ray crystallographic data up to 2.08 A resolution have been collected from these crystals.
AB - PAB0955 from Pyrococcus abyssi is a prototype of a new Walker-type ATPase/GTPase conserved in archaea and eukaryota but not found in bacteria. PAB0955 has been expressed, purified and crystallized, and it has been shown that this thermostable protein is dimeric in reductive conditions. Crystals have been obtained either without nucleotide or in the presence of GDP or GTPgammaS. Preliminary X-ray crystallographic data up to 2.08 A resolution have been collected from these crystals.
UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1952251/pdf/f-61-00208.pdf
U2 - 10.1107/S1744309105000035
DO - 10.1107/S1744309105000035
M3 - Article
SN - 1744-3091
VL - 61
SP - 208
EP - 211
JO - Acta Crystallographica Section F: Structural Biology Communications
JF - Acta Crystallographica Section F: Structural Biology Communications
IS - Pt 2
ER -