Expression, purification, crystallization and preliminary crystallographic analysis of the PAB0955 gene product

Stephanie Gras, Bernard Fernandez, Valerie Chaumont, Philippe Carpentier, Jean Armengaud, Dominique Housset

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Abstract

PAB0955 from Pyrococcus abyssi is a prototype of a new Walker-type ATPase/GTPase conserved in archaea and eukaryota but not found in bacteria. PAB0955 has been expressed, purified and crystallized, and it has been shown that this thermostable protein is dimeric in reductive conditions. Crystals have been obtained either without nucleotide or in the presence of GDP or GTPgammaS. Preliminary X-ray crystallographic data up to 2.08 A resolution have been collected from these crystals.
Original languageEnglish
Pages (from-to)208 - 211
Number of pages4
Journal Acta Crystallographica Section F: Structural Biology Communications
Volume61
Issue numberPt 2
DOIs
Publication statusPublished - 2005
Externally publishedYes

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