Expression patterns and roles of periostin during kidney and ureter development

Katrina Sorocos, Xenia Kostoulias, Luise Cullen-McEwen, Adam Hart, John Bertram, Georgina Caruana

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

Periostin is a secreted extracellular matrix protein that is differentially expressed in the developing kidney. We analyzed the temporal-spatial expression of periostin in the developing kidney and ureter as well as its roles in ureter branching morphogenesis, nephrogenesis and ureter development. MATERIALS AND METHODS: RNA in situ hybridization and immunofluorescence histochemistry were used to investigate the expression of periostin, alphav integrin and alpha-smooth muscle actin during mouse renal and ureteral development. Metanephric explants were cultured in the presence of recombinant periostin, and ureteral branch points/tips and the glomerular number were quantified. Explants were also cultured in the presence of exogenous bone morphogenetic protein 4 and the effect on periostin mRNA levels was determined by quantitative real-time polymerase chain reaction. RESULTS: Periostin expression was observed in the mesenchyme surrounding the kidney and ureter, renal stroma, metanephric mesenchyme, ureter epithelium and developing nephrons. At embryonic day 15.5 periostin and alphav integrin, a common subunit of periostin receptors, were co-expressed in smooth muscle cells of the ureter, renal artery and intrarenal arteries. Bone morphogenetic protein 4 up-regulated periostin mRNA expression and exogenous periostin inhibited branching morphogenesis and glomerular number. CONCLUSIONS: Bone morphogenetic protein 4 which inhibits ureteral branching morphogenesis and promotes smooth muscle cell migration in the ureter up-regulated periostin mRNA expression in the developing kidney. Ureteral smooth muscle cells express periostin and alphav integrin. Periostin inhibited ureteral branching morphogenesis and glomerular number. Together these results suggest that periostin and bone morphogenetic protein 4 may have a role in branching morphogenesis, nephrogenesis and possibly smooth muscle cell migration.
Original languageEnglish
Pages (from-to)1537 - 1544
Number of pages8
JournalJournal of Urology
Volume186
Issue number4
DOIs
Publication statusPublished - 2011

Cite this

Sorocos, Katrina ; Kostoulias, Xenia ; Cullen-McEwen, Luise ; Hart, Adam ; Bertram, John ; Caruana, Georgina. / Expression patterns and roles of periostin during kidney and ureter development. In: Journal of Urology. 2011 ; Vol. 186, No. 4. pp. 1537 - 1544.
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abstract = "Periostin is a secreted extracellular matrix protein that is differentially expressed in the developing kidney. We analyzed the temporal-spatial expression of periostin in the developing kidney and ureter as well as its roles in ureter branching morphogenesis, nephrogenesis and ureter development. MATERIALS AND METHODS: RNA in situ hybridization and immunofluorescence histochemistry were used to investigate the expression of periostin, alphav integrin and alpha-smooth muscle actin during mouse renal and ureteral development. Metanephric explants were cultured in the presence of recombinant periostin, and ureteral branch points/tips and the glomerular number were quantified. Explants were also cultured in the presence of exogenous bone morphogenetic protein 4 and the effect on periostin mRNA levels was determined by quantitative real-time polymerase chain reaction. RESULTS: Periostin expression was observed in the mesenchyme surrounding the kidney and ureter, renal stroma, metanephric mesenchyme, ureter epithelium and developing nephrons. At embryonic day 15.5 periostin and alphav integrin, a common subunit of periostin receptors, were co-expressed in smooth muscle cells of the ureter, renal artery and intrarenal arteries. Bone morphogenetic protein 4 up-regulated periostin mRNA expression and exogenous periostin inhibited branching morphogenesis and glomerular number. CONCLUSIONS: Bone morphogenetic protein 4 which inhibits ureteral branching morphogenesis and promotes smooth muscle cell migration in the ureter up-regulated periostin mRNA expression in the developing kidney. Ureteral smooth muscle cells express periostin and alphav integrin. Periostin inhibited ureteral branching morphogenesis and glomerular number. Together these results suggest that periostin and bone morphogenetic protein 4 may have a role in branching morphogenesis, nephrogenesis and possibly smooth muscle cell migration.",
author = "Katrina Sorocos and Xenia Kostoulias and Luise Cullen-McEwen and Adam Hart and John Bertram and Georgina Caruana",
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Expression patterns and roles of periostin during kidney and ureter development. / Sorocos, Katrina; Kostoulias, Xenia; Cullen-McEwen, Luise; Hart, Adam; Bertram, John; Caruana, Georgina.

In: Journal of Urology, Vol. 186, No. 4, 2011, p. 1537 - 1544.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Expression patterns and roles of periostin during kidney and ureter development

AU - Sorocos, Katrina

AU - Kostoulias, Xenia

AU - Cullen-McEwen, Luise

AU - Hart, Adam

AU - Bertram, John

AU - Caruana, Georgina

PY - 2011

Y1 - 2011

N2 - Periostin is a secreted extracellular matrix protein that is differentially expressed in the developing kidney. We analyzed the temporal-spatial expression of periostin in the developing kidney and ureter as well as its roles in ureter branching morphogenesis, nephrogenesis and ureter development. MATERIALS AND METHODS: RNA in situ hybridization and immunofluorescence histochemistry were used to investigate the expression of periostin, alphav integrin and alpha-smooth muscle actin during mouse renal and ureteral development. Metanephric explants were cultured in the presence of recombinant periostin, and ureteral branch points/tips and the glomerular number were quantified. Explants were also cultured in the presence of exogenous bone morphogenetic protein 4 and the effect on periostin mRNA levels was determined by quantitative real-time polymerase chain reaction. RESULTS: Periostin expression was observed in the mesenchyme surrounding the kidney and ureter, renal stroma, metanephric mesenchyme, ureter epithelium and developing nephrons. At embryonic day 15.5 periostin and alphav integrin, a common subunit of periostin receptors, were co-expressed in smooth muscle cells of the ureter, renal artery and intrarenal arteries. Bone morphogenetic protein 4 up-regulated periostin mRNA expression and exogenous periostin inhibited branching morphogenesis and glomerular number. CONCLUSIONS: Bone morphogenetic protein 4 which inhibits ureteral branching morphogenesis and promotes smooth muscle cell migration in the ureter up-regulated periostin mRNA expression in the developing kidney. Ureteral smooth muscle cells express periostin and alphav integrin. Periostin inhibited ureteral branching morphogenesis and glomerular number. Together these results suggest that periostin and bone morphogenetic protein 4 may have a role in branching morphogenesis, nephrogenesis and possibly smooth muscle cell migration.

AB - Periostin is a secreted extracellular matrix protein that is differentially expressed in the developing kidney. We analyzed the temporal-spatial expression of periostin in the developing kidney and ureter as well as its roles in ureter branching morphogenesis, nephrogenesis and ureter development. MATERIALS AND METHODS: RNA in situ hybridization and immunofluorescence histochemistry were used to investigate the expression of periostin, alphav integrin and alpha-smooth muscle actin during mouse renal and ureteral development. Metanephric explants were cultured in the presence of recombinant periostin, and ureteral branch points/tips and the glomerular number were quantified. Explants were also cultured in the presence of exogenous bone morphogenetic protein 4 and the effect on periostin mRNA levels was determined by quantitative real-time polymerase chain reaction. RESULTS: Periostin expression was observed in the mesenchyme surrounding the kidney and ureter, renal stroma, metanephric mesenchyme, ureter epithelium and developing nephrons. At embryonic day 15.5 periostin and alphav integrin, a common subunit of periostin receptors, were co-expressed in smooth muscle cells of the ureter, renal artery and intrarenal arteries. Bone morphogenetic protein 4 up-regulated periostin mRNA expression and exogenous periostin inhibited branching morphogenesis and glomerular number. CONCLUSIONS: Bone morphogenetic protein 4 which inhibits ureteral branching morphogenesis and promotes smooth muscle cell migration in the ureter up-regulated periostin mRNA expression in the developing kidney. Ureteral smooth muscle cells express periostin and alphav integrin. Periostin inhibited ureteral branching morphogenesis and glomerular number. Together these results suggest that periostin and bone morphogenetic protein 4 may have a role in branching morphogenesis, nephrogenesis and possibly smooth muscle cell migration.

UR - http://www.ncbi.nlm.nih.gov/pubmed/21855915

U2 - 10.1016/j.juro.2011.05.042

DO - 10.1016/j.juro.2011.05.042

M3 - Article

VL - 186

SP - 1537

EP - 1544

JO - Journal of Urology

JF - Journal of Urology

SN - 0022-5347

IS - 4

ER -