Expression of two conserved leptospiral antigens in Escherichia coli

J. P. Doherty; B. Adler; J. I. Rood; S. J. Billington; S. Faine

doi:10.1099/00222615-28-2-143

Expression of two conserved leptospiral antigens in Escherichia coli

J. P. Doherty, B. Adler, J. I. Rood, S. J. Billington, S. Faine

Microbiology

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9 Citations (Scopus)

Abstract

The genes encoding two protein antigens of Leptospira interrogans serovar pomona were cloned and expressed in Escherichia coli. Rabbit antisera raised against the cloned proteins, designated p12 and p20, were used to identify the antigens in Western blots of disrupted leptospiral cells. The proteins p12 and p20 were conserved within the genus Leptospira and were not detected in Leptonema illini. Although both proteins were present in leptospiral outer envelope preparations they did not elicit the production of agglutinating or opsonising antibodies.

Original language	English
Pages (from-to)	143-149
Number of pages	7
Journal	Journal of Medical Microbiology
Volume	28
Issue number	2
DOIs	https://doi.org/10.1099/00222615-28-2-143
Publication status	Published - 1 Jan 1989

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AU - Doherty, J. P.

AU - Adler, B.

AU - Rood, J. I.

AU - Billington, S. J.

AU - Faine, S.

PY - 1989/1/1

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AB - The genes encoding two protein antigens of Leptospira interrogans serovar pomona were cloned and expressed in Escherichia coli. Rabbit antisera raised against the cloned proteins, designated p12 and p20, were used to identify the antigens in Western blots of disrupted leptospiral cells. The proteins p12 and p20 were conserved within the genus Leptospira and were not detected in Leptonema illini. Although both proteins were present in leptospiral outer envelope preparations they did not elicit the production of agglutinating or opsonising antibodies.

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Expression of two conserved leptospiral antigens in Escherichia coli

Abstract

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