Abstract
B-cell chronic lymphocytic leukaemia (CLL) cells commonly express the multidrug resistance phenotype. The aim of this study was to establish whether the normal homologue in B-cell ontogeny of B-CLL also expressed the multidrug resistance (mdr) phenotype. Human tonsillar lymphocytes were sorted to yield two B-cell subsets based on the expression of CD19, CD5 and CD10. The normal homologue was represented by a population of B cells that was CD19 positive, CD10 negative and weakly expressed CD5. Based upon functional analysis and the detection of mdr1 mRNA by semi-quantitative PCR, these cells expressed the mdr phenotype. In contrast, functional multidrug resistace could not be demonstrated in CD19-positive CD10-positive cells with strong expression of CD5, nor could mdr1 mRNA be found in these cells. MRP was variably expressed in both B-cell subsets with no discernable differences in the pattern of expression. We conclude that normal B cells with a phenotype resembling that of B-CLL cells express the multidrug resistace phenotype.
| Original language | English |
|---|---|
| Pages (from-to) | 697-707 |
| Number of pages | 11 |
| Journal | British Journal of Haematology |
| Volume | 96 |
| Issue number | 4 |
| Publication status | Published - 1997 |
| Externally published | Yes |
Keywords
- B-CLL
- mdr1
- normal B-cell homologues
Cite this
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Expression of mdr1 and mrp in the normal B-cell homologue of B-cell chronic lymphocytic leukaemia. / Wall, D. M P; El-Osta, S.; Tzelepis, D.; Bertoncello, I.; Kantharidis, P.; Chou, S. T.; Zalcberg, J. R.; Parkin, J. D.
In: British Journal of Haematology, Vol. 96, No. 4, 1997, p. 697-707.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Expression of mdr1 and mrp in the normal B-cell homologue of B-cell chronic lymphocytic leukaemia
AU - Wall, D. M P
AU - El-Osta, S.
AU - Tzelepis, D.
AU - Bertoncello, I.
AU - Kantharidis, P.
AU - Chou, S. T.
AU - Zalcberg, J. R.
AU - Parkin, J. D.
PY - 1997
Y1 - 1997
N2 - B-cell chronic lymphocytic leukaemia (CLL) cells commonly express the multidrug resistance phenotype. The aim of this study was to establish whether the normal homologue in B-cell ontogeny of B-CLL also expressed the multidrug resistance (mdr) phenotype. Human tonsillar lymphocytes were sorted to yield two B-cell subsets based on the expression of CD19, CD5 and CD10. The normal homologue was represented by a population of B cells that was CD19 positive, CD10 negative and weakly expressed CD5. Based upon functional analysis and the detection of mdr1 mRNA by semi-quantitative PCR, these cells expressed the mdr phenotype. In contrast, functional multidrug resistace could not be demonstrated in CD19-positive CD10-positive cells with strong expression of CD5, nor could mdr1 mRNA be found in these cells. MRP was variably expressed in both B-cell subsets with no discernable differences in the pattern of expression. We conclude that normal B cells with a phenotype resembling that of B-CLL cells express the multidrug resistace phenotype.
AB - B-cell chronic lymphocytic leukaemia (CLL) cells commonly express the multidrug resistance phenotype. The aim of this study was to establish whether the normal homologue in B-cell ontogeny of B-CLL also expressed the multidrug resistance (mdr) phenotype. Human tonsillar lymphocytes were sorted to yield two B-cell subsets based on the expression of CD19, CD5 and CD10. The normal homologue was represented by a population of B cells that was CD19 positive, CD10 negative and weakly expressed CD5. Based upon functional analysis and the detection of mdr1 mRNA by semi-quantitative PCR, these cells expressed the mdr phenotype. In contrast, functional multidrug resistace could not be demonstrated in CD19-positive CD10-positive cells with strong expression of CD5, nor could mdr1 mRNA be found in these cells. MRP was variably expressed in both B-cell subsets with no discernable differences in the pattern of expression. We conclude that normal B cells with a phenotype resembling that of B-CLL cells express the multidrug resistace phenotype.
KW - B-CLL
KW - mdr1
KW - normal B-cell homologues
UR - http://www.scopus.com/inward/record.url?scp=0031049270&partnerID=8YFLogxK
M3 - Article
VL - 96
SP - 697
EP - 707
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 4
ER -