Expression of mdr1 and mrp in the normal B-cell homologue of B-cell chronic lymphocytic leukaemia

D. M P Wall, S. El-Osta, D. Tzelepis, I. Bertoncello, P. Kantharidis, S. T. Chou, J. R. Zalcberg, J. D. Parkin

Research output: Contribution to journalArticleResearchpeer-review

Abstract

B-cell chronic lymphocytic leukaemia (CLL) cells commonly express the multidrug resistance phenotype. The aim of this study was to establish whether the normal homologue in B-cell ontogeny of B-CLL also expressed the multidrug resistance (mdr) phenotype. Human tonsillar lymphocytes were sorted to yield two B-cell subsets based on the expression of CD19, CD5 and CD10. The normal homologue was represented by a population of B cells that was CD19 positive, CD10 negative and weakly expressed CD5. Based upon functional analysis and the detection of mdr1 mRNA by semi-quantitative PCR, these cells expressed the mdr phenotype. In contrast, functional multidrug resistace could not be demonstrated in CD19-positive CD10-positive cells with strong expression of CD5, nor could mdr1 mRNA be found in these cells. MRP was variably expressed in both B-cell subsets with no discernable differences in the pattern of expression. We conclude that normal B cells with a phenotype resembling that of B-CLL cells express the multidrug resistace phenotype.

Original languageEnglish
Pages (from-to)697-707
Number of pages11
JournalBritish Journal of Haematology
Volume96
Issue number4
Publication statusPublished - 1997
Externally publishedYes

Keywords

  • B-CLL
  • mdr1
  • normal B-cell homologues

Cite this

Wall, D. M. P., El-Osta, S., Tzelepis, D., Bertoncello, I., Kantharidis, P., Chou, S. T., ... Parkin, J. D. (1997). Expression of mdr1 and mrp in the normal B-cell homologue of B-cell chronic lymphocytic leukaemia. British Journal of Haematology, 96(4), 697-707.
Wall, D. M P ; El-Osta, S. ; Tzelepis, D. ; Bertoncello, I. ; Kantharidis, P. ; Chou, S. T. ; Zalcberg, J. R. ; Parkin, J. D. / Expression of mdr1 and mrp in the normal B-cell homologue of B-cell chronic lymphocytic leukaemia. In: British Journal of Haematology. 1997 ; Vol. 96, No. 4. pp. 697-707.
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abstract = "B-cell chronic lymphocytic leukaemia (CLL) cells commonly express the multidrug resistance phenotype. The aim of this study was to establish whether the normal homologue in B-cell ontogeny of B-CLL also expressed the multidrug resistance (mdr) phenotype. Human tonsillar lymphocytes were sorted to yield two B-cell subsets based on the expression of CD19, CD5 and CD10. The normal homologue was represented by a population of B cells that was CD19 positive, CD10 negative and weakly expressed CD5. Based upon functional analysis and the detection of mdr1 mRNA by semi-quantitative PCR, these cells expressed the mdr phenotype. In contrast, functional multidrug resistace could not be demonstrated in CD19-positive CD10-positive cells with strong expression of CD5, nor could mdr1 mRNA be found in these cells. MRP was variably expressed in both B-cell subsets with no discernable differences in the pattern of expression. We conclude that normal B cells with a phenotype resembling that of B-CLL cells express the multidrug resistace phenotype.",
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Expression of mdr1 and mrp in the normal B-cell homologue of B-cell chronic lymphocytic leukaemia. / Wall, D. M P; El-Osta, S.; Tzelepis, D.; Bertoncello, I.; Kantharidis, P.; Chou, S. T.; Zalcberg, J. R.; Parkin, J. D.

In: British Journal of Haematology, Vol. 96, No. 4, 1997, p. 697-707.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Expression of mdr1 and mrp in the normal B-cell homologue of B-cell chronic lymphocytic leukaemia

AU - Wall, D. M P

AU - El-Osta, S.

AU - Tzelepis, D.

AU - Bertoncello, I.

AU - Kantharidis, P.

AU - Chou, S. T.

AU - Zalcberg, J. R.

AU - Parkin, J. D.

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AB - B-cell chronic lymphocytic leukaemia (CLL) cells commonly express the multidrug resistance phenotype. The aim of this study was to establish whether the normal homologue in B-cell ontogeny of B-CLL also expressed the multidrug resistance (mdr) phenotype. Human tonsillar lymphocytes were sorted to yield two B-cell subsets based on the expression of CD19, CD5 and CD10. The normal homologue was represented by a population of B cells that was CD19 positive, CD10 negative and weakly expressed CD5. Based upon functional analysis and the detection of mdr1 mRNA by semi-quantitative PCR, these cells expressed the mdr phenotype. In contrast, functional multidrug resistace could not be demonstrated in CD19-positive CD10-positive cells with strong expression of CD5, nor could mdr1 mRNA be found in these cells. MRP was variably expressed in both B-cell subsets with no discernable differences in the pattern of expression. We conclude that normal B cells with a phenotype resembling that of B-CLL cells express the multidrug resistace phenotype.

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