Abstract
Objective: To define the stage-by-stage expression of KATNB1 during human spermatogenesis. Design: Gene expression analysis, histologic and immunohistochemical evaluation. Setting: University research laboratories and andrological clinic. Patient(s): Eighty human testicular biopsy samples: 43 showing normal spermatogenesis, 9 with maturation arrest at level of spermatocytes, 8 with maturation arrest at level of spermatogonia, and 20 with a Sertoli cell only syndrome. Intervention(s): None. Main Outcome Measure(s): Evaluation of katanin p80 expression in normal as well as impaired spermatogenesis on mRNA (RT-PCR, RT-qPCR, and in situ hybridization) and protein level (immunohistochemistry/immunofluorescence). Result(s): KATNB1 messenger RNA is exclusively expressed in germ cells, and quantitatively reduced in maturation arrests at the level of spermatogonia. The KATNB1 protein was detected in type B spermatogonia entering meiosis and in the Golgi complex of pachytene spermatocytes. Immediately before the first meiotic division, it is colocalized with the cleaving centriole. It was also detected in early round spermatids in the dictyosome. Conclusion(s): The expression and localization of KATNB1 support a role in spindle formation. The localization of KATNB1 in early round spermatids suggests an involvement in the formation of microtubule-based structures during spermiogenesis (manchette and flagellum). These data are consistent with the demonstrated role of KATNB1 in mouse meiosis, nuclear shaping, and flagellum formation of sperm and suggest the strong conservation of function even between distantly related species.
| Original language | English |
|---|---|
| Pages (from-to) | 1683-1690 |
| Number of pages | 8 |
| Journal | Fertility and Sterility |
| Volume | 106 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Dec 2016 |
Keywords
- Katanin p80
- meiosis
- microtubules
- spermatogenesis
- spermiogenesis
Cite this
}
Expression of katanin p80 in human spermatogenesis. / Pleuger, Christiane; Fietz, Daniela; Hartmann, Katja; Weidner, Wolfgang; Kliesch, Sabine; O'Bryan, Moira K.; Dorresteign, Adriaan; Bergmann, Martin.
In: Fertility and Sterility, Vol. 106, No. 7, 12.2016, p. 1683-1690.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Expression of katanin p80 in human spermatogenesis
AU - Pleuger, Christiane
AU - Fietz, Daniela
AU - Hartmann, Katja
AU - Weidner, Wolfgang
AU - Kliesch, Sabine
AU - O'Bryan, Moira K.
AU - Dorresteign, Adriaan
AU - Bergmann, Martin
PY - 2016/12
Y1 - 2016/12
N2 - Objective: To define the stage-by-stage expression of KATNB1 during human spermatogenesis. Design: Gene expression analysis, histologic and immunohistochemical evaluation. Setting: University research laboratories and andrological clinic. Patient(s): Eighty human testicular biopsy samples: 43 showing normal spermatogenesis, 9 with maturation arrest at level of spermatocytes, 8 with maturation arrest at level of spermatogonia, and 20 with a Sertoli cell only syndrome. Intervention(s): None. Main Outcome Measure(s): Evaluation of katanin p80 expression in normal as well as impaired spermatogenesis on mRNA (RT-PCR, RT-qPCR, and in situ hybridization) and protein level (immunohistochemistry/immunofluorescence). Result(s): KATNB1 messenger RNA is exclusively expressed in germ cells, and quantitatively reduced in maturation arrests at the level of spermatogonia. The KATNB1 protein was detected in type B spermatogonia entering meiosis and in the Golgi complex of pachytene spermatocytes. Immediately before the first meiotic division, it is colocalized with the cleaving centriole. It was also detected in early round spermatids in the dictyosome. Conclusion(s): The expression and localization of KATNB1 support a role in spindle formation. The localization of KATNB1 in early round spermatids suggests an involvement in the formation of microtubule-based structures during spermiogenesis (manchette and flagellum). These data are consistent with the demonstrated role of KATNB1 in mouse meiosis, nuclear shaping, and flagellum formation of sperm and suggest the strong conservation of function even between distantly related species.
AB - Objective: To define the stage-by-stage expression of KATNB1 during human spermatogenesis. Design: Gene expression analysis, histologic and immunohistochemical evaluation. Setting: University research laboratories and andrological clinic. Patient(s): Eighty human testicular biopsy samples: 43 showing normal spermatogenesis, 9 with maturation arrest at level of spermatocytes, 8 with maturation arrest at level of spermatogonia, and 20 with a Sertoli cell only syndrome. Intervention(s): None. Main Outcome Measure(s): Evaluation of katanin p80 expression in normal as well as impaired spermatogenesis on mRNA (RT-PCR, RT-qPCR, and in situ hybridization) and protein level (immunohistochemistry/immunofluorescence). Result(s): KATNB1 messenger RNA is exclusively expressed in germ cells, and quantitatively reduced in maturation arrests at the level of spermatogonia. The KATNB1 protein was detected in type B spermatogonia entering meiosis and in the Golgi complex of pachytene spermatocytes. Immediately before the first meiotic division, it is colocalized with the cleaving centriole. It was also detected in early round spermatids in the dictyosome. Conclusion(s): The expression and localization of KATNB1 support a role in spindle formation. The localization of KATNB1 in early round spermatids suggests an involvement in the formation of microtubule-based structures during spermiogenesis (manchette and flagellum). These data are consistent with the demonstrated role of KATNB1 in mouse meiosis, nuclear shaping, and flagellum formation of sperm and suggest the strong conservation of function even between distantly related species.
KW - Katanin p80
KW - meiosis
KW - microtubules
KW - spermatogenesis
KW - spermiogenesis
UR - http://www.scopus.com/inward/record.url?scp=84999025055&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2016.08.043
DO - 10.1016/j.fertnstert.2016.08.043
M3 - Article
VL - 106
SP - 1683
EP - 1690
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 7
ER -