Expression of katanin p80 in human spermatogenesis

Christiane Pleuger, Daniela Fietz, Katja Hartmann, Wolfgang Weidner, Sabine Kliesch, Moira K. O'Bryan, Adriaan Dorresteign, Martin Bergmann

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Objective: To define the stage-by-stage expression of KATNB1 during human spermatogenesis. Design: Gene expression analysis, histologic and immunohistochemical evaluation. Setting: University research laboratories and andrological clinic. Patient(s): Eighty human testicular biopsy samples: 43 showing normal spermatogenesis, 9 with maturation arrest at level of spermatocytes, 8 with maturation arrest at level of spermatogonia, and 20 with a Sertoli cell only syndrome. Intervention(s): None. Main Outcome Measure(s): Evaluation of katanin p80 expression in normal as well as impaired spermatogenesis on mRNA (RT-PCR, RT-qPCR, and in situ hybridization) and protein level (immunohistochemistry/immunofluorescence). Result(s): KATNB1 messenger RNA is exclusively expressed in germ cells, and quantitatively reduced in maturation arrests at the level of spermatogonia. The KATNB1 protein was detected in type B spermatogonia entering meiosis and in the Golgi complex of pachytene spermatocytes. Immediately before the first meiotic division, it is colocalized with the cleaving centriole. It was also detected in early round spermatids in the dictyosome. Conclusion(s): The expression and localization of KATNB1 support a role in spindle formation. The localization of KATNB1 in early round spermatids suggests an involvement in the formation of microtubule-based structures during spermiogenesis (manchette and flagellum). These data are consistent with the demonstrated role of KATNB1 in mouse meiosis, nuclear shaping, and flagellum formation of sperm and suggest the strong conservation of function even between distantly related species.

Original languageEnglish
Pages (from-to)1683-1690
Number of pages8
JournalFertility and Sterility
Volume106
Issue number7
DOIs
Publication statusPublished - Dec 2016

Keywords

  • Katanin p80
  • meiosis
  • microtubules
  • spermatogenesis
  • spermiogenesis

Cite this

Pleuger, C., Fietz, D., Hartmann, K., Weidner, W., Kliesch, S., O'Bryan, M. K., ... Bergmann, M. (2016). Expression of katanin p80 in human spermatogenesis. Fertility and Sterility, 106(7), 1683-1690. https://doi.org/10.1016/j.fertnstert.2016.08.043
Pleuger, Christiane ; Fietz, Daniela ; Hartmann, Katja ; Weidner, Wolfgang ; Kliesch, Sabine ; O'Bryan, Moira K. ; Dorresteign, Adriaan ; Bergmann, Martin. / Expression of katanin p80 in human spermatogenesis. In: Fertility and Sterility. 2016 ; Vol. 106, No. 7. pp. 1683-1690.
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Pleuger, C, Fietz, D, Hartmann, K, Weidner, W, Kliesch, S, O'Bryan, MK, Dorresteign, A & Bergmann, M 2016, 'Expression of katanin p80 in human spermatogenesis' Fertility and Sterility, vol. 106, no. 7, pp. 1683-1690. https://doi.org/10.1016/j.fertnstert.2016.08.043

Expression of katanin p80 in human spermatogenesis. / Pleuger, Christiane; Fietz, Daniela; Hartmann, Katja; Weidner, Wolfgang; Kliesch, Sabine; O'Bryan, Moira K.; Dorresteign, Adriaan; Bergmann, Martin.

In: Fertility and Sterility, Vol. 106, No. 7, 12.2016, p. 1683-1690.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Pleuger, Christiane

AU - Fietz, Daniela

AU - Hartmann, Katja

AU - Weidner, Wolfgang

AU - Kliesch, Sabine

AU - O'Bryan, Moira K.

AU - Dorresteign, Adriaan

AU - Bergmann, Martin

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Pleuger C, Fietz D, Hartmann K, Weidner W, Kliesch S, O'Bryan MK et al. Expression of katanin p80 in human spermatogenesis. Fertility and Sterility. 2016 Dec;106(7):1683-1690. https://doi.org/10.1016/j.fertnstert.2016.08.043