Abstract
DNA derived from regions upstream of the Salmonella enterica serovar Typhimurium ssaG gene were used to drive expression of different reporter genes in putative Salmonella vaccine strains. Expression from ssaG was shown to be significantly upregulated once Salmonella had entered murine or human macrophages, and levels of expression were dependent on the length of the ssaG 5′ sequence incorporated. S. Typhimurium derivatives harbouring the Escherichia coli heat labile toxin B subunit (LT-B) fused to various lengths of the ssaG promoter region were also constructed as single copy chromosomal integrations. Expression of LT-B by these Salmonella derivatives was detected at significant levels after intra-macrophage survival and mice immunised with these derivatives mounted marked anti-LT-B humoral antibody responses.
Original language | English |
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Pages (from-to) | 3243-3255 |
Number of pages | 13 |
Journal | Vaccine |
Volume | 22 |
Issue number | 25-26 |
DOIs | |
Publication status | Published - 3 Sept 2004 |
Externally published | Yes |
Keywords
- Chromosomal expression
- E. coli heat labile toxin
- In vivo-inducible expression
- S. Typhimurium
- Salmonella Pathogenicity Island-2
- ssaG promoter