Expression of Cre Recombinase in the developing mouse limb bud driven by a Prxl enhancer

Malcolm Logan, James F. Martin, Andras Nagy, Corrinne Lobe, Eric N. Olson, Clifford J. Tabin

Research output: Contribution to journalArticleResearchpeer-review

537 Citations (Scopus)

Abstract

We have used a Prx1 limb enhancer to drive expression of Cre Recombinase in transgenic mice. This regulatory element leads to Cre expression throughout the early limb bud mesenchyme and in a subset of craniofacial mesenchyme. Crossing a murine line carrying this transgene to a reporter mouse harboring a floxed Cre-reporter cassette revealed that recombinase activity is first observed in the earliest limb bud at 9.5 dpc. By early to mid bud stages at 10.5 dpc recombination is essentially complete in all mesenchymal cells in the limb. Expression of the Cre recombinase was never detected in the limb bud ectoderm. The use of Prx1-Cre mice should facilitate analysis of gene function in the developing limb.

Original languageEnglish
Pages (from-to)77-80
Number of pages4
Journalgenesis: The Journal of Genetics and Development
Volume33
Issue number2
DOIs
Publication statusPublished - 2002
Externally publishedYes

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