Expression of caveolin by bovine lymphocytes and antigen-presenting cells

James Harris, Dirk Werling, Michael Koss, Paul Monaghan, Geraldine Taylor, Chris J. Howard

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Caveolin is a generic term for a family of proteins that include caveolin-1, -2 and -3. Although the distribution of these proteins varies between cells, caveolin-1 and -2 are commonly found coating membrane invaginations known as caveolae. Studies on human and murine cells suggest that caveolin/caveolae can be found in neutrophils, macrophages and mast cells, in which they are involved in the uptake of pathogens, but not in lymphocyte cell lines. Expression of caveolin-1, -2 and -3 in bovine immune cells was investigated using confocal microscopy and Western blotting. Staining for caveolin-1 was evident in all peripheral blood mononuclear cells (PBMC) and in CD4+, CD8+ and CD21+ lymphocytes, monocytes, macrophages and monocyte-derived dendritic cells (DC). In addition, the caveolin-1 antibody detected a protein with a molecular weight of approximately 22000 in all PBMC, macrophages and DC, as well as in bovine aortic endothelial (BAE)-I cells and human endothelial cells by Western blotting. In macrophages and DC, caveolin co-localized with the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and to a lesser extent with Golgi, but not with endoplasmic reticulum. Staining was not seen on the plasma membrane in any bovine immune cells, suggesting the absence of caveolae, while in BAE-1 cells staining was predominantly on the cell membrane. Caveolin-2 could not be detected in any bovine cells by confocal microscopy or Western blotting, while caveolin-3 was detected in all bovine cells by Western blotting, but not by confocal microscopy. These data provide evidence for the presence of caveolin in bovine lymphocytes and antigen-presenting cells.

Original languageEnglish
Pages (from-to)190-195
Number of pages6
JournalImmunology
Volume105
Issue number2
DOIs
Publication statusPublished - 2002
Externally publishedYes

Cite this

Harris, J., Werling, D., Koss, M., Monaghan, P., Taylor, G., & Howard, C. J. (2002). Expression of caveolin by bovine lymphocytes and antigen-presenting cells. Immunology, 105(2), 190-195. https://doi.org/10.1046/j.1365-2567.2002.01362.x
Harris, James ; Werling, Dirk ; Koss, Michael ; Monaghan, Paul ; Taylor, Geraldine ; Howard, Chris J. / Expression of caveolin by bovine lymphocytes and antigen-presenting cells. In: Immunology. 2002 ; Vol. 105, No. 2. pp. 190-195.
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abstract = "Caveolin is a generic term for a family of proteins that include caveolin-1, -2 and -3. Although the distribution of these proteins varies between cells, caveolin-1 and -2 are commonly found coating membrane invaginations known as caveolae. Studies on human and murine cells suggest that caveolin/caveolae can be found in neutrophils, macrophages and mast cells, in which they are involved in the uptake of pathogens, but not in lymphocyte cell lines. Expression of caveolin-1, -2 and -3 in bovine immune cells was investigated using confocal microscopy and Western blotting. Staining for caveolin-1 was evident in all peripheral blood mononuclear cells (PBMC) and in CD4+, CD8+ and CD21+ lymphocytes, monocytes, macrophages and monocyte-derived dendritic cells (DC). In addition, the caveolin-1 antibody detected a protein with a molecular weight of approximately 22000 in all PBMC, macrophages and DC, as well as in bovine aortic endothelial (BAE)-I cells and human endothelial cells by Western blotting. In macrophages and DC, caveolin co-localized with the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and to a lesser extent with Golgi, but not with endoplasmic reticulum. Staining was not seen on the plasma membrane in any bovine immune cells, suggesting the absence of caveolae, while in BAE-1 cells staining was predominantly on the cell membrane. Caveolin-2 could not be detected in any bovine cells by confocal microscopy or Western blotting, while caveolin-3 was detected in all bovine cells by Western blotting, but not by confocal microscopy. These data provide evidence for the presence of caveolin in bovine lymphocytes and antigen-presenting cells.",
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Harris, J, Werling, D, Koss, M, Monaghan, P, Taylor, G & Howard, CJ 2002, 'Expression of caveolin by bovine lymphocytes and antigen-presenting cells' Immunology, vol. 105, no. 2, pp. 190-195. https://doi.org/10.1046/j.1365-2567.2002.01362.x

Expression of caveolin by bovine lymphocytes and antigen-presenting cells. / Harris, James; Werling, Dirk; Koss, Michael; Monaghan, Paul; Taylor, Geraldine; Howard, Chris J.

In: Immunology, Vol. 105, No. 2, 2002, p. 190-195.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Expression of caveolin by bovine lymphocytes and antigen-presenting cells

AU - Harris, James

AU - Werling, Dirk

AU - Koss, Michael

AU - Monaghan, Paul

AU - Taylor, Geraldine

AU - Howard, Chris J.

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