Expression of a gene for glucan-binding protein from Streptococcus mutans in Escherichia coli

R. R.B. Russell; D  Coleman; G. Dougan

Expression of a gene for glucan-binding protein from Streptococcus mutans in Escherichia coli

R. R.B. Russell, D Coleman, G. Dougan

Research output: Contribution to journal › Article › Research › peer-review

Abstract

The structural gene for a glucan-binding protein (GBP) of Streptococcus mutans has been inserted into a bacteriophage λ vector and expressed in Escherichia coli K12. Lysates of E. coli infected with the recombinant phage contain an antigenic protein of the same size as S. mutans GBP. The GBP synthesized in E. coli can be affinity-purified on immobilized glucan and antiserum raised against it has been shown to precipitate fructosyltransferase activity from S. mutans.

Original language	English
Pages (from-to)	295-299
Number of pages	5
Journal	Journal of General Microbiology
Volume	131
Issue number	2
Publication status	Published - 1 Jan 1985
Externally published	Yes

Cite this

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abstract = "The structural gene for a glucan-binding protein (GBP) of Streptococcus mutans has been inserted into a bacteriophage λ vector and expressed in Escherichia coli K12. Lysates of E. coli infected with the recombinant phage contain an antigenic protein of the same size as S. mutans GBP. The GBP synthesized in E. coli can be affinity-purified on immobilized glucan and antiserum raised against it has been shown to precipitate fructosyltransferase activity from S. mutans.",

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N2 - The structural gene for a glucan-binding protein (GBP) of Streptococcus mutans has been inserted into a bacteriophage λ vector and expressed in Escherichia coli K12. Lysates of E. coli infected with the recombinant phage contain an antigenic protein of the same size as S. mutans GBP. The GBP synthesized in E. coli can be affinity-purified on immobilized glucan and antiserum raised against it has been shown to precipitate fructosyltransferase activity from S. mutans.

AB - The structural gene for a glucan-binding protein (GBP) of Streptococcus mutans has been inserted into a bacteriophage λ vector and expressed in Escherichia coli K12. Lysates of E. coli infected with the recombinant phage contain an antigenic protein of the same size as S. mutans GBP. The GBP synthesized in E. coli can be affinity-purified on immobilized glucan and antiserum raised against it has been shown to precipitate fructosyltransferase activity from S. mutans.

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Expression of a gene for glucan-binding protein from Streptococcus mutans in Escherichia coli

Abstract

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