Expression of a chemically synthesized human α1 interferon gene

E. de Maeyer, D. Skup, K. S.N. Prasad, J. De Maeyer-Guignard, B. Williams, P. Meacock, G. Sharpe, D. Pioli, J. Hennam, W. Schuch, K. Atherton

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Cells of Escherichia coli containing a chemically synthesized human α1 interferon (IFN-α1) gene, under control of the lac promoter, make a product with biological properties indistinguishable from those of the natural IFN-α1 [antiviral activity, acid stability, species crossreactivity, inactivation by antisera directed against leukocyte or Namalwa cell interferon, and stimulation of (2'-5')oligoadenylate synthetase activity]. Similar levels of IFN synthesis were obtained when the expression unit (lac promoter plus synthetic IFN-α1 gene) was transplanted into the obligate methylotroph Methylophilus methylnotrophus.

Original languageEnglish
Pages (from-to)4256-4259
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number14 I
Publication statusPublished - 1 Dec 1982
Externally publishedYes

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