Cells of Escherichia coli containing a chemically synthesized human α1 interferon (IFN-α1) gene, under control of the lac promoter, make a product with biological properties indistinguishable from those of the natural IFN-α1 [antiviral activity, acid stability, species crossreactivity, inactivation by antisera directed against leukocyte or Namalwa cell interferon, and stimulation of (2'-5')oligoadenylate synthetase activity]. Similar levels of IFN synthesis were obtained when the expression unit (lac promoter plus synthetic IFN-α1 gene) was transplanted into the obligate methylotroph Methylophilus methylnotrophus.
|Number of pages||4|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|Issue number||14 I|
|Publication status||Published - 1 Dec 1982|