Expression in yeast of antisense RNA to ADE1 mRNA.

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Abstract

The utility of antisense RNA as a means of regulating gene expression in yeast has been explored by inserting into a high copy number yeast expression vector an ADE1 gene fragment in such an orientation so as to produce antisense RNA in vivo which could hybridize to natural ADE1 mRNA. Northern blotting analysis of total cellular RNA extracted from transformed yeast cells confirmed the presence of high levels of antisense RNA to ADE1 mRNA within cells. However the high level of expression of antisense RNA did not result in production of Ade- cells.

Original languageEnglish
Pages (from-to)673-679
Number of pages7
JournalBiochemistry International
Volume17
Issue number4
Publication statusPublished - 1 Jan 1988

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