Expression feature of CD3, Fc{epsilon}RI{gamma}, and Zap-70 in patients with chronic lymphocytic leukemia

Le Huang, Shaohua Chen, Xianfeng Zha, Lijian Yang, Bo Li, Zhi Yu, Liang Wang, Yangqiu Li

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13 Citations (Scopus)

Abstract

In leukemia patients, T-cell function has been suppressed with the disease progress. Patients with chronic lymphocytic leukemia (CLL) are all to a degree immunodeficient. In order to elucidate the feature of T-cell receptor signal transduction in CLL, the expression levels of CD3?, d, e, and ? chain, FceRI?, and Zap-70 genes in peripheral blood mononuclear cells (PBMCs) were analyzed. Real-time polymerase chain reaction with SYBR Green technique was used for detecting the gene expression level in PBMCs from 13 patients with CLL, 13 healthy individuals, and 10 B-cell acute lymphocytic leukemia (B-ALL) served as control. The ?2-microglobulin gene was used as an endogenous reference. Relative mRNA expression level of genes was analyzed by using the 2-?Ct ? 100 method. Significant lower expression levels of CD3?, e, and ? chain genes, as well as FceRI? gene were found in CLL samples. Moreover, there was lost the negative correlation of the expression levels between CD3? and FceRI? genes. The expression level of Zap-70 in CLL was lower than those from healthy controls, while higher than those from B-ALL group. There was no significant correlation between the expression levels of CD3? and Zap-70 genes neither in the healthy group nor in the CLL group. In conclusion, the results provide a global gene expression profile of CD3?, d, e, and ? chains, and the CD3?-related genes FceRI? and Zap-70 in CLL. Deficiency of these gene expression levels might represent the feature related to T-cell immunodeficiency. The study might contribute to better understand the cellular immune features in CLL patients.
Original languageEnglish
Pages (from-to)71 - 75
Number of pages5
JournalHematology
Volume17
Issue number2
DOIs
Publication statusPublished - 2012

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