Expression cloning of a cDNA encoding the type II regulatory subunit of the cAMP-dependent protein kinase

Brian A. Hemmings, Manuela Schwarz, S. Rao Adavani, David A. Jans

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Abstract

We report here the isolation and sequence of a cDNA for the type II regulatory subunit of the cAMP-dependent protein kinase (cAMP-PK) from a λgt-11 cDNA library derived from a porcine epithelial cell line (LLC-PK1). The cDNA was detected by immunological screening using an affinity purified polyclonal antibody for bovine RII. DNA sequence analysis of the 467 bp EcoRI insert confirmed the identity of the clone, because the deduced amino acid sequence corresponded to the published sequence for the bovine RΠ protein. Northern analysis of total RNA from the LLC-PK1 cells indicated a single mRNA species of about 6.0 kb, probably derived from a single copy gene.

Original languageEnglish
Pages (from-to)219-222
Number of pages4
JournalFEBS Letters
Volume209
Issue number2
DOIs
Publication statusPublished - 15 Dec 1986
Externally publishedYes

Keywords

  • (LLC-PK cell)
  • cyclic AMP dependence
  • Expression cloning
  • Protein kinase
  • Regulatory subunit

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