Expression and characterization of a minimal hepatitis C virus glycoprotein E2 core domain that retains CD81 binding

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The hepatitis C virus glycoprotein E2 receptor-binding domain is encompassed by amino acids 384 to 661 (E2 661) and contains two hypervariable sequences, HVR1 and HVR2. E2 sequence comparisons revealed a third variable region, located between residues 570 and 580, that varies widely between genotypes, designated here as igVR, the intergenotypic variable region. A secreted E2 661 glycoprotein with simultaneous deletions of the three variable sequences retained its ability to bind CD81 and conformation-dependent monoclonal antibodies (MAbs) and displayed enhanced binding to a neutralizing MAb directed to E2 immunogenic domain B. Our data provide insights into the E2 structure by suggesting that the three variable regions reside outside a conserved E2 core.

Original languageEnglish
Pages (from-to)9584-9590
Number of pages7
JournalJournal of Virology
Issue number17
Publication statusPublished - 1 Sep 2007
Externally publishedYes

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