Expressed sequence tag analysis and development of gene associated markers in a near-isogenic plant system of Eragrostis curvula

Gerardo D L Cervigni, Norma Paniego, Marina Diaz, Juan Pablo Selva, Diego Zappacosta, Dario Zanazzi, Inaki Landerreche, Luciano Gaston Martelotto, Silvina Felitti, Silvina C Pessino, German C Spangenberg, Viviana Echenique

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29 Citations (Scopus)

Abstract

Eragrostis curvula (Schrad.) Nees is a forage grass native to the semiarid regions of Southern Africa, which reproduces mainly by pseudogamous diplosporous apomixis. A collection of ESTs was generated from four cDNA libraries, three of them obtained from panicles of near-isogenic lines with different ploidy levels and reproductive modes, and one obtained from 12 days-old plant leaves. A total of 12,295 high-quality ESTs were clustered and assembled, rendering 8,864 unigenes, including 1,490 contigs and 7,394 singletons, with a genome coverage of 22 . A total of 7,029 (79.11 ) unigenes were functionally categorized by BLASTX analysis against sequences deposited in public databases, but only 37.80 could be classified according to Gene Ontology. Sequence comparison against the cereals genes indexes (GI) revealed 50 significant hits. A total of 254 EST-SSRs were detected from 219 singletons and 35 from contigs. Di- and tri- motifs were similarly represented with percentages of 38.95 and 40.16 , respectively. In addition, 190 SNPs and Indels were detected in 18 contigs generated from 3 to 4 libraries. The ESTs and the molecular markers obtained in this study will provide valuable resources for a wide range of applications including gene identification, genetic mapping, cultivar identification, analysis of genetic diversity, phenotype mapping and marker assisted selection.
Original languageEnglish
Pages (from-to)1 - 10
Number of pages10
JournalPlant Molecular Biology
Volume67
Issue number1-2
DOIs
Publication statusPublished - 2008
Externally publishedYes

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