Exploring potential Germline-Associated roles of the TRIM-NHL Protein NHL-2 through RNAi screening

Gregory M. Davis, Wai Y. Low, Joshua W. T. Anderson, Peter R. Boag

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

TRIM-NHL proteins are highly conserved regulators of developmental pathways in vertebrates and invertebrates. The TRIM-NHL family member NHL-2 in Caenorhabditis elegans functions as a miRNA cofactor to regulate developmental timing. Similar regulatory roles have been reported in other model systems, with the mammalian ortholog in mice, TRIM32, contributing to muscle and neuronal cell proliferation via miRNA activity. Given the interest associated with TRIM-NHL family proteins, we aimed to further investigate the role of NHL-2 in C. elegans development by using a synthetic RNAi screening approach. Using the ORFeome library, we knocked down 11,942 genes in wild-type animals and nhl-2 null mutants. In total, we identified 42 genes that produced strong reproductive synthetic phenotypes when knocked down in nhl-2 null mutants, with little or no change when knocked down in wild-type animals. These included genes associated with transcriptional processes, chromosomal integrity, and key cofactors of the germline small 22G RNA pathway.

Original languageEnglish
Pages (from-to)3251-3256
Number of pages6
JournalG3: Genes, Genomes, Genetics
Volume7
Issue number10
DOIs
Publication statusPublished - 1 Oct 2017

Keywords

  • Caenorhabditis
  • Elegans
  • Germline
  • Mutant Screen
  • NHL-2
  • Report
  • RNAi screen
  • TRIM-NHL

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