Exploring Appetite and Hypothalamic Circuitry through Manipulating Gene Expression

Tara Jois, Mark W. Sleeman

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

This chapter overviews the approaches used by researchers in the neuroendocrinology field with a specific focus on the neuroendocrine control of food intake. It also emphasizes principles and recent developments. The chapter reviews all transgenic models of neuroendocrine systems that impact control of appetite and body weight regulation. Inbred mouse strains have become an important tool for disease related medical research and extensive phenotyping has shown genetic diversity both across strains and within strains. The study of global germ line deletions has provided important functional information for neuropeptides and their receptors, and offered validation of numerous pathways for ligand-mediated behaviors involved in feeding and body weight regulation. Recently, the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system has gained attention as a tool for inducing targeted genetic alterations much more quickly than other available technologies. We also have other complex set of tools for functionalizing the genome, such as gene on/off systems.

Original languageEnglish
Title of host publicationNeuroendocrinology of Appetite
EditorsSuzanne L. Dickson, Julian G. Mercer
Place of PublicationUnited Kingdom
PublisherWiley-Blackwell
Pages134-150
Number of pages17
ISBN (Electronic)9781118839317
ISBN (Print)9781118839324
DOIs
Publication statusPublished - 10 Aug 2016

Keywords

  • Appetite control
  • Arcuate nucleus
  • Cas mediated genome engineering
  • CRISPR system
  • Food intake control
  • Neuroendocrinology
  • Pro-opiomelanocortin
  • Transgenic technology

Cite this

Jois, T., & Sleeman, M. W. (2016). Exploring Appetite and Hypothalamic Circuitry through Manipulating Gene Expression. In S. L. Dickson, & J. G. Mercer (Eds.), Neuroendocrinology of Appetite (pp. 134-150). Wiley-Blackwell. https://doi.org/10.1002/9781118839317.ch7