Exploration of mechanisms in nutriepigenomics: Identification of chromatin-modifying compounds from Olea Europaea

Natalie P. Bonvino, Nancy B. Ray, Vi T. Luu, Julia Liang, Andrew Hung, Tom Karagiannis

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Chemical modification of histones represents an important epigenetic mechanism critical for DNA metabolism including,transcription, replication and repair. A well-known example is maintenance of histone acetylation status by the opposing actions of histone acetyltransferase and histone deacetylase enzymes which add and remove acetyl groups on lysine residues on histone tails, respectively. Similarly, histone methyltransferase and histone demethylase enzymes are responsible for adding and removing methyl groups on histone tails, respectively. Further, there is accumulated evidence indicating a histone code where combinations of different chemical modifications on histone tails act in concert to regulate DNA metabolic events.Although numerous compounds have been developed to specifically alter the function of chromatin modifying enzymes (for example, histone deacetylase inhibitors are relatively well-investigated), we are only at the early stages of understanding the epigenetic effects of dietary compounds. Here we used in silico molecular modeling approaches combined with known experimental affinities for controls, to identify potential chromatin modifying compounds derived from Olea Europaea. Our findings indicate that various compounds derived from Olea Europaea have the ability to bind to the active site of different chromatin modifying enzymes, with an affinity analogous or higher than that for a known positive control. Further, we initiated the process of validating targets using in vitro binding and enzyme activity inhibition assays and provide initial findings of potential epigenetic effects in a clinical context. Overall, our findings can be considered as the first instalment of a comprehensive endeavour to catalogue and detail the epigenetic effects of compounds derived from Olea Europaea. HJNM 2015; 18(Suppl1); 51-62
Original languageEnglish
Pages (from-to)51-62
Number of pages12
JournalHellenic Journal of Nuclear Medicine
Volume18
Issue numberSuppl 1
Publication statusPublished - Sep 2015
Externally publishedYes

Keywords

  • Olive oil
  • Olive phenolics
  • Epigenetics
  • Histone acetylation
  • Histone methylation

Cite this

Bonvino, Natalie P. ; Ray, Nancy B. ; Luu, Vi T. ; Liang, Julia ; Hung, Andrew ; Karagiannis, Tom. / Exploration of mechanisms in nutriepigenomics : Identification of chromatin-modifying compounds from Olea Europaea. In: Hellenic Journal of Nuclear Medicine. 2015 ; Vol. 18, No. Suppl 1. pp. 51-62.
@article{16919c08cfb34c89a108bf73b92460b3,
title = "Exploration of mechanisms in nutriepigenomics: Identification of chromatin-modifying compounds from Olea Europaea",
abstract = "Chemical modification of histones represents an important epigenetic mechanism critical for DNA metabolism including,transcription, replication and repair. A well-known example is maintenance of histone acetylation status by the opposing actions of histone acetyltransferase and histone deacetylase enzymes which add and remove acetyl groups on lysine residues on histone tails, respectively. Similarly, histone methyltransferase and histone demethylase enzymes are responsible for adding and removing methyl groups on histone tails, respectively. Further, there is accumulated evidence indicating a histone code where combinations of different chemical modifications on histone tails act in concert to regulate DNA metabolic events.Although numerous compounds have been developed to specifically alter the function of chromatin modifying enzymes (for example, histone deacetylase inhibitors are relatively well-investigated), we are only at the early stages of understanding the epigenetic effects of dietary compounds. Here we used in silico molecular modeling approaches combined with known experimental affinities for controls, to identify potential chromatin modifying compounds derived from Olea Europaea. Our findings indicate that various compounds derived from Olea Europaea have the ability to bind to the active site of different chromatin modifying enzymes, with an affinity analogous or higher than that for a known positive control. Further, we initiated the process of validating targets using in vitro binding and enzyme activity inhibition assays and provide initial findings of potential epigenetic effects in a clinical context. Overall, our findings can be considered as the first instalment of a comprehensive endeavour to catalogue and detail the epigenetic effects of compounds derived from Olea Europaea. HJNM 2015; 18(Suppl1); 51-62",
keywords = "Olive oil, Olive phenolics, Epigenetics, Histone acetylation, Histone methylation",
author = "Bonvino, {Natalie P.} and Ray, {Nancy B.} and Luu, {Vi T.} and Julia Liang and Andrew Hung and Tom Karagiannis",
year = "2015",
month = "9",
language = "English",
volume = "18",
pages = "51--62",
journal = "Hellenic Journal of Nuclear Medicine",
issn = "1790-5427",
number = "Suppl 1",

}

Exploration of mechanisms in nutriepigenomics : Identification of chromatin-modifying compounds from Olea Europaea. / Bonvino, Natalie P.; Ray, Nancy B.; Luu, Vi T.; Liang, Julia; Hung, Andrew; Karagiannis, Tom.

In: Hellenic Journal of Nuclear Medicine, Vol. 18, No. Suppl 1, 09.2015, p. 51-62.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Exploration of mechanisms in nutriepigenomics

T2 - Identification of chromatin-modifying compounds from Olea Europaea

AU - Bonvino, Natalie P.

AU - Ray, Nancy B.

AU - Luu, Vi T.

AU - Liang, Julia

AU - Hung, Andrew

AU - Karagiannis, Tom

PY - 2015/9

Y1 - 2015/9

N2 - Chemical modification of histones represents an important epigenetic mechanism critical for DNA metabolism including,transcription, replication and repair. A well-known example is maintenance of histone acetylation status by the opposing actions of histone acetyltransferase and histone deacetylase enzymes which add and remove acetyl groups on lysine residues on histone tails, respectively. Similarly, histone methyltransferase and histone demethylase enzymes are responsible for adding and removing methyl groups on histone tails, respectively. Further, there is accumulated evidence indicating a histone code where combinations of different chemical modifications on histone tails act in concert to regulate DNA metabolic events.Although numerous compounds have been developed to specifically alter the function of chromatin modifying enzymes (for example, histone deacetylase inhibitors are relatively well-investigated), we are only at the early stages of understanding the epigenetic effects of dietary compounds. Here we used in silico molecular modeling approaches combined with known experimental affinities for controls, to identify potential chromatin modifying compounds derived from Olea Europaea. Our findings indicate that various compounds derived from Olea Europaea have the ability to bind to the active site of different chromatin modifying enzymes, with an affinity analogous or higher than that for a known positive control. Further, we initiated the process of validating targets using in vitro binding and enzyme activity inhibition assays and provide initial findings of potential epigenetic effects in a clinical context. Overall, our findings can be considered as the first instalment of a comprehensive endeavour to catalogue and detail the epigenetic effects of compounds derived from Olea Europaea. HJNM 2015; 18(Suppl1); 51-62

AB - Chemical modification of histones represents an important epigenetic mechanism critical for DNA metabolism including,transcription, replication and repair. A well-known example is maintenance of histone acetylation status by the opposing actions of histone acetyltransferase and histone deacetylase enzymes which add and remove acetyl groups on lysine residues on histone tails, respectively. Similarly, histone methyltransferase and histone demethylase enzymes are responsible for adding and removing methyl groups on histone tails, respectively. Further, there is accumulated evidence indicating a histone code where combinations of different chemical modifications on histone tails act in concert to regulate DNA metabolic events.Although numerous compounds have been developed to specifically alter the function of chromatin modifying enzymes (for example, histone deacetylase inhibitors are relatively well-investigated), we are only at the early stages of understanding the epigenetic effects of dietary compounds. Here we used in silico molecular modeling approaches combined with known experimental affinities for controls, to identify potential chromatin modifying compounds derived from Olea Europaea. Our findings indicate that various compounds derived from Olea Europaea have the ability to bind to the active site of different chromatin modifying enzymes, with an affinity analogous or higher than that for a known positive control. Further, we initiated the process of validating targets using in vitro binding and enzyme activity inhibition assays and provide initial findings of potential epigenetic effects in a clinical context. Overall, our findings can be considered as the first instalment of a comprehensive endeavour to catalogue and detail the epigenetic effects of compounds derived from Olea Europaea. HJNM 2015; 18(Suppl1); 51-62

KW - Olive oil

KW - Olive phenolics

KW - Epigenetics

KW - Histone acetylation

KW - Histone methylation

UR - http://www.nuclmed.web.auth.gr/magazine/eng/sept15/suppl.pdf

M3 - Article

VL - 18

SP - 51

EP - 62

JO - Hellenic Journal of Nuclear Medicine

JF - Hellenic Journal of Nuclear Medicine

SN - 1790-5427

IS - Suppl 1

ER -