Experimental tools for the study of protein phosphorylation in plasmodium

Dominique Dorin-Semblat; Andrew R Bottrill; Lev Solyakov; Andrew Tobin; Christian Daniel Doerig

doi:10.1007/978-1-62703-026-7

Experimental tools for the study of protein phosphorylation in plasmodium

Dominique Dorin-Semblat, Andrew R Bottrill, Lev Solyakov, Andrew Tobin, Christian Daniel Doerig

Microbiology

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Research › peer-review

6 Citations (Scopus)

Abstract

The central role played by protein phosphorylation in the regulation of eukaryotic cellular processes calls for detailed investigations of this phenomenon in malaria parasites. Here, we describe protocols to measure the activity of protein kinases (using either recombinant proteins or native enzymes purified from parasite extracts), and outline procedures to identify phosphorylation sites on parasite proteins following a mass spectrometry approach.

Original language	English
Title of host publication	Malaria
Subtitle of host publication	Methods and Protocols
Editors	Robert Menard
Place of Publication	USA
Publisher	Humana Press
Pages	241-257
Number of pages	17
Edition	2nd
ISBN (Print)	9781627030250
DOIs	https://doi.org/10.1007/978-1-62703-026-7
Publication status	Published - 1 Jan 2013

Publication series

Name	Methods in Molecular Biology
Volume	923
ISSN (Print)	1064-3745

Keywords

Kinase assay
Phosphoproteomics
Phosphorylation
Protein kinase

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-62703-026-7

Cite this

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Experimental tools for the study of protein phosphorylation in plasmodium. / Dorin-Semblat, Dominique; Bottrill, Andrew R; Solyakov, Lev et al.
Malaria: Methods and Protocols. ed. / Robert Menard. 2nd. ed. USA: Humana Press, 2013. p. 241-257 (Methods in Molecular Biology; Vol. 923).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Research › peer-review

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AU - Bottrill, Andrew R

AU - Solyakov, Lev

AU - Tobin, Andrew

AU - Doerig, Christian Daniel

PY - 2013/1/1

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AB - The central role played by protein phosphorylation in the regulation of eukaryotic cellular processes calls for detailed investigations of this phenomenon in malaria parasites. Here, we describe protocols to measure the activity of protein kinases (using either recombinant proteins or native enzymes purified from parasite extracts), and outline procedures to identify phosphorylation sites on parasite proteins following a mass spectrometry approach.

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KW - Phosphoproteomics

KW - Phosphorylation

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Experimental tools for the study of protein phosphorylation in plasmodium

Abstract

Publication series

Keywords

UN SDGs

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