Experimental tools for the study of protein phosphorylation in plasmodium

Dominique Dorin-Semblat, Andrew R Bottrill, Lev Solyakov, Andrew Tobin, Christian Daniel Doerig

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

6 Citations (Scopus)


The central role played by protein phosphorylation in the regulation of eukaryotic cellular processes calls for detailed investigations of this phenomenon in malaria parasites. Here, we describe protocols to measure the activity of protein kinases (using either recombinant proteins or native enzymes purified from parasite extracts), and outline procedures to identify phosphorylation sites on parasite proteins following a mass spectrometry approach.

Original languageEnglish
Title of host publicationMalaria
Subtitle of host publicationMethods and Protocols
EditorsRobert Menard
Place of PublicationUSA
PublisherHumana Press
Number of pages17
ISBN (Print)9781627030250
Publication statusPublished - 1 Jan 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Kinase assay
  • Phosphoproteomics
  • Phosphorylation
  • Protein kinase

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