Experimental intrauterine Ureaplasma infection in sheep

Timothy Moss, Ilias Nitsos, Machiko Ikegami, Alan Jobe, John Newnham

Research output: Contribution to journalArticleResearchpeer-review

Abstract

OBJECTIVE: Prenatal Ureaplasma spp exposure is associated with preterm birth and modulates the neonates susceptibility to respiratory distress syndrome and bronchopulmonary dysplasia. We hypothesized that intra-amniotic ureaplasmas would cause lung inflammation and alter fetal lung development. STUDY DESIGN: Pregnant ewes bearing singleton fetuses were given an intra-amniotic injection of 20 x 10 6 CFUs of U parvum (serovar 3) or vehicle, either 1, 3, 6, or 10 weeks before the delivery of preterm lambs at 124 days of gestation (n = 4-10 per group) for evaluation of inflammation and fetal lung maturation. RESULTS: Ureaplasmas were recovered from amniotic and fetal lung fluids after intra-amniotic injection. Body weight and umbilical arterial pH were reduced by Ureaplasma exposure for 10 weeks ( P <.05). Ureaplasmas caused progressive lung inflammation and improvements in lung function that were associated with increased surfactant lipids (control, 0.13 +/- 0.02 micromol/kg; 10 weeks of Ureaplasma exposure, 7.43 +/- 3.0 micromol/kg; P <.001) and surfactant protein messenger RNA expression. CONCLUSION: Long-term exposure to ureaplasmas in amniotic fluid alters ovine fetal development.
Original languageEnglish
Pages (from-to)1179 - 1186
Number of pages8
JournalAmerican Journal of Obstetrics and Gynecology
Volume192
Issue number4
DOIs
Publication statusPublished - 2005
Externally publishedYes

Cite this

Moss, Timothy ; Nitsos, Ilias ; Ikegami, Machiko ; Jobe, Alan ; Newnham, John. / Experimental intrauterine Ureaplasma infection in sheep. In: American Journal of Obstetrics and Gynecology. 2005 ; Vol. 192, No. 4. pp. 1179 - 1186.
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title = "Experimental intrauterine Ureaplasma infection in sheep",
abstract = "OBJECTIVE: Prenatal Ureaplasma spp exposure is associated with preterm birth and modulates the neonates susceptibility to respiratory distress syndrome and bronchopulmonary dysplasia. We hypothesized that intra-amniotic ureaplasmas would cause lung inflammation and alter fetal lung development. STUDY DESIGN: Pregnant ewes bearing singleton fetuses were given an intra-amniotic injection of 20 x 10 6 CFUs of U parvum (serovar 3) or vehicle, either 1, 3, 6, or 10 weeks before the delivery of preterm lambs at 124 days of gestation (n = 4-10 per group) for evaluation of inflammation and fetal lung maturation. RESULTS: Ureaplasmas were recovered from amniotic and fetal lung fluids after intra-amniotic injection. Body weight and umbilical arterial pH were reduced by Ureaplasma exposure for 10 weeks ( P <.05). Ureaplasmas caused progressive lung inflammation and improvements in lung function that were associated with increased surfactant lipids (control, 0.13 +/- 0.02 micromol/kg; 10 weeks of Ureaplasma exposure, 7.43 +/- 3.0 micromol/kg; P <.001) and surfactant protein messenger RNA expression. CONCLUSION: Long-term exposure to ureaplasmas in amniotic fluid alters ovine fetal development.",
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Experimental intrauterine Ureaplasma infection in sheep. / Moss, Timothy; Nitsos, Ilias; Ikegami, Machiko; Jobe, Alan; Newnham, John.

In: American Journal of Obstetrics and Gynecology, Vol. 192, No. 4, 2005, p. 1179 - 1186.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Experimental intrauterine Ureaplasma infection in sheep

AU - Moss, Timothy

AU - Nitsos, Ilias

AU - Ikegami, Machiko

AU - Jobe, Alan

AU - Newnham, John

PY - 2005

Y1 - 2005

N2 - OBJECTIVE: Prenatal Ureaplasma spp exposure is associated with preterm birth and modulates the neonates susceptibility to respiratory distress syndrome and bronchopulmonary dysplasia. We hypothesized that intra-amniotic ureaplasmas would cause lung inflammation and alter fetal lung development. STUDY DESIGN: Pregnant ewes bearing singleton fetuses were given an intra-amniotic injection of 20 x 10 6 CFUs of U parvum (serovar 3) or vehicle, either 1, 3, 6, or 10 weeks before the delivery of preterm lambs at 124 days of gestation (n = 4-10 per group) for evaluation of inflammation and fetal lung maturation. RESULTS: Ureaplasmas were recovered from amniotic and fetal lung fluids after intra-amniotic injection. Body weight and umbilical arterial pH were reduced by Ureaplasma exposure for 10 weeks ( P <.05). Ureaplasmas caused progressive lung inflammation and improvements in lung function that were associated with increased surfactant lipids (control, 0.13 +/- 0.02 micromol/kg; 10 weeks of Ureaplasma exposure, 7.43 +/- 3.0 micromol/kg; P <.001) and surfactant protein messenger RNA expression. CONCLUSION: Long-term exposure to ureaplasmas in amniotic fluid alters ovine fetal development.

AB - OBJECTIVE: Prenatal Ureaplasma spp exposure is associated with preterm birth and modulates the neonates susceptibility to respiratory distress syndrome and bronchopulmonary dysplasia. We hypothesized that intra-amniotic ureaplasmas would cause lung inflammation and alter fetal lung development. STUDY DESIGN: Pregnant ewes bearing singleton fetuses were given an intra-amniotic injection of 20 x 10 6 CFUs of U parvum (serovar 3) or vehicle, either 1, 3, 6, or 10 weeks before the delivery of preterm lambs at 124 days of gestation (n = 4-10 per group) for evaluation of inflammation and fetal lung maturation. RESULTS: Ureaplasmas were recovered from amniotic and fetal lung fluids after intra-amniotic injection. Body weight and umbilical arterial pH were reduced by Ureaplasma exposure for 10 weeks ( P <.05). Ureaplasmas caused progressive lung inflammation and improvements in lung function that were associated with increased surfactant lipids (control, 0.13 +/- 0.02 micromol/kg; 10 weeks of Ureaplasma exposure, 7.43 +/- 3.0 micromol/kg; P <.001) and surfactant protein messenger RNA expression. CONCLUSION: Long-term exposure to ureaplasmas in amniotic fluid alters ovine fetal development.

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