Experimental and simulation studies of a real-time polymerase chain reaction in the presence of a fullerene derivative

Junjun Shang, Tatsiana A. Ratnikova, Sini Anttalainen, Emppu Salonen, Pu Chun Ke, Halina T. Knap

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Abstract

The real-time polymerase chain reaction of the plant gene heat shock transcription factor was fully inhibited in the presence of a fullerene derivative C60(OH)20 at a concentration of 4 × 10-4mM. This inhibition was attributed to the interaction between the nanoparticle and Taq DNA polymerase in the reaction. Atomistic molecular dynamics simulations showed a clear tendency for hydrogen bonding between C 60(OH)20 and both the dNTPs and ssDNA components of the polymerase chain reaction. These studies facilitate our understanding of the fate of nanoparticles in biomolecular systems, a topic of tremendous importance for addressing the biological and environmental implications of nanomaterials.

Original languageEnglish
Article number415101
JournalNanotechnology
Volume20
Issue number41
DOIs
Publication statusPublished - 2009
Externally publishedYes

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