Experimental and simulation studies of a real-time polymerase chain reaction in the presence of a fullerene derivative

Junjun Shang, Tatsiana A. Ratnikova, Sini Anttalainen, Emppu Salonen, Pu Chun Ke, Halina T. Knap

Research output: Contribution to journalArticleResearchpeer-review

23 Citations (Scopus)


The real-time polymerase chain reaction of the plant gene heat shock transcription factor was fully inhibited in the presence of a fullerene derivative C60(OH)20 at a concentration of 4 × 10-4mM. This inhibition was attributed to the interaction between the nanoparticle and Taq DNA polymerase in the reaction. Atomistic molecular dynamics simulations showed a clear tendency for hydrogen bonding between C 60(OH)20 and both the dNTPs and ssDNA components of the polymerase chain reaction. These studies facilitate our understanding of the fate of nanoparticles in biomolecular systems, a topic of tremendous importance for addressing the biological and environmental implications of nanomaterials.

Original languageEnglish
Article number415101
Issue number41
Publication statusPublished - 2009
Externally publishedYes

Cite this