Experimental amniotic fluid infection in sheep: Effects of Ureaplasma parvum serovars 3 and 6 on preterm or term fetal sheep

Timothy Moss, Christine Knox, Suhas Kallapur, Ilias Nitsos, Christina Theodoropoulos, John Newnham, Machiko Ikegami, Alan Jobe

Research output: Contribution to journalArticleResearchpeer-review

Abstract

OBJECTIVE: The objective of the study was to determine the effects in late gestation of Ureaplasma parvum serovar 3 colonization and the effects, preterm, of U. parvum serovar 6. STUDY DESIGN: Ewes received an intraamniotic (i.a.) injection of U. parvum serovar 6 (20 x 10(6) colony-forming units [cfu]; n = 9), U. parvum serovar 3 (20 x 10(3) cfu; n = 6), vehicle (n = 10), or saline (n = 4) on day 80 of pregnancy (d). The lambs were delivered at 125 d (U. parvum serovar 6, n = 9; saline or media controls, n = 9) or 145 d (U. parvum serovar 3, n = 6; media controls, n = 5) for assessment of inflammation and lung maturation. RESULTS: I.a. ureaplasmas caused histologic chorioamnionitis but not preterm delivery. Fetal lung epithelium was colonized with ureaplasmas at both gestational ages, and pulmonary interleukin-8 levels had doubled in the ureaplasma-colonized animals, compared with the controls at 145 d. Surfactant levels in bronchoalveolar lavage fluid had increased 8-fold and 2.5-fold at 125 and 145 d, respectively, after ureaplasma injection. CONCLUSION: Fetal lung inflammation and altered development accompanies ureaplasma colonization, regardless of age at delivery.
Original languageEnglish
Pages (from-to)122.e1 - 122.e8
Number of pages8
JournalAmerican Journal of Obstetrics and Gynecology
Volume198
Issue number1
DOIs
Publication statusPublished - 2008
Externally publishedYes

Cite this

Moss, Timothy ; Knox, Christine ; Kallapur, Suhas ; Nitsos, Ilias ; Theodoropoulos, Christina ; Newnham, John ; Ikegami, Machiko ; Jobe, Alan. / Experimental amniotic fluid infection in sheep: Effects of Ureaplasma parvum serovars 3 and 6 on preterm or term fetal sheep. In: American Journal of Obstetrics and Gynecology. 2008 ; Vol. 198, No. 1. pp. 122.e1 - 122.e8.
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title = "Experimental amniotic fluid infection in sheep: Effects of Ureaplasma parvum serovars 3 and 6 on preterm or term fetal sheep",
abstract = "OBJECTIVE: The objective of the study was to determine the effects in late gestation of Ureaplasma parvum serovar 3 colonization and the effects, preterm, of U. parvum serovar 6. STUDY DESIGN: Ewes received an intraamniotic (i.a.) injection of U. parvum serovar 6 (20 x 10(6) colony-forming units [cfu]; n = 9), U. parvum serovar 3 (20 x 10(3) cfu; n = 6), vehicle (n = 10), or saline (n = 4) on day 80 of pregnancy (d). The lambs were delivered at 125 d (U. parvum serovar 6, n = 9; saline or media controls, n = 9) or 145 d (U. parvum serovar 3, n = 6; media controls, n = 5) for assessment of inflammation and lung maturation. RESULTS: I.a. ureaplasmas caused histologic chorioamnionitis but not preterm delivery. Fetal lung epithelium was colonized with ureaplasmas at both gestational ages, and pulmonary interleukin-8 levels had doubled in the ureaplasma-colonized animals, compared with the controls at 145 d. Surfactant levels in bronchoalveolar lavage fluid had increased 8-fold and 2.5-fold at 125 and 145 d, respectively, after ureaplasma injection. CONCLUSION: Fetal lung inflammation and altered development accompanies ureaplasma colonization, regardless of age at delivery.",
author = "Timothy Moss and Christine Knox and Suhas Kallapur and Ilias Nitsos and Christina Theodoropoulos and John Newnham and Machiko Ikegami and Alan Jobe",
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Experimental amniotic fluid infection in sheep: Effects of Ureaplasma parvum serovars 3 and 6 on preterm or term fetal sheep. / Moss, Timothy; Knox, Christine; Kallapur, Suhas; Nitsos, Ilias; Theodoropoulos, Christina; Newnham, John; Ikegami, Machiko; Jobe, Alan.

In: American Journal of Obstetrics and Gynecology, Vol. 198, No. 1, 2008, p. 122.e1 - 122.e8.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Experimental amniotic fluid infection in sheep: Effects of Ureaplasma parvum serovars 3 and 6 on preterm or term fetal sheep

AU - Moss, Timothy

AU - Knox, Christine

AU - Kallapur, Suhas

AU - Nitsos, Ilias

AU - Theodoropoulos, Christina

AU - Newnham, John

AU - Ikegami, Machiko

AU - Jobe, Alan

PY - 2008

Y1 - 2008

N2 - OBJECTIVE: The objective of the study was to determine the effects in late gestation of Ureaplasma parvum serovar 3 colonization and the effects, preterm, of U. parvum serovar 6. STUDY DESIGN: Ewes received an intraamniotic (i.a.) injection of U. parvum serovar 6 (20 x 10(6) colony-forming units [cfu]; n = 9), U. parvum serovar 3 (20 x 10(3) cfu; n = 6), vehicle (n = 10), or saline (n = 4) on day 80 of pregnancy (d). The lambs were delivered at 125 d (U. parvum serovar 6, n = 9; saline or media controls, n = 9) or 145 d (U. parvum serovar 3, n = 6; media controls, n = 5) for assessment of inflammation and lung maturation. RESULTS: I.a. ureaplasmas caused histologic chorioamnionitis but not preterm delivery. Fetal lung epithelium was colonized with ureaplasmas at both gestational ages, and pulmonary interleukin-8 levels had doubled in the ureaplasma-colonized animals, compared with the controls at 145 d. Surfactant levels in bronchoalveolar lavage fluid had increased 8-fold and 2.5-fold at 125 and 145 d, respectively, after ureaplasma injection. CONCLUSION: Fetal lung inflammation and altered development accompanies ureaplasma colonization, regardless of age at delivery.

AB - OBJECTIVE: The objective of the study was to determine the effects in late gestation of Ureaplasma parvum serovar 3 colonization and the effects, preterm, of U. parvum serovar 6. STUDY DESIGN: Ewes received an intraamniotic (i.a.) injection of U. parvum serovar 6 (20 x 10(6) colony-forming units [cfu]; n = 9), U. parvum serovar 3 (20 x 10(3) cfu; n = 6), vehicle (n = 10), or saline (n = 4) on day 80 of pregnancy (d). The lambs were delivered at 125 d (U. parvum serovar 6, n = 9; saline or media controls, n = 9) or 145 d (U. parvum serovar 3, n = 6; media controls, n = 5) for assessment of inflammation and lung maturation. RESULTS: I.a. ureaplasmas caused histologic chorioamnionitis but not preterm delivery. Fetal lung epithelium was colonized with ureaplasmas at both gestational ages, and pulmonary interleukin-8 levels had doubled in the ureaplasma-colonized animals, compared with the controls at 145 d. Surfactant levels in bronchoalveolar lavage fluid had increased 8-fold and 2.5-fold at 125 and 145 d, respectively, after ureaplasma injection. CONCLUSION: Fetal lung inflammation and altered development accompanies ureaplasma colonization, regardless of age at delivery.

UR - http://www.sciencedirect.com/science/article/pii/S0002937807008411

U2 - 10.1016/j.ajog.2007.06.065

DO - 10.1016/j.ajog.2007.06.065

M3 - Article

VL - 198

SP - 122.e1 - 122.e8

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 1

ER -