Research output per year
Research output per year
Abhilasha Tiwari, Melinda L Tursky, Lakshmi Prasanna Nekkanti, Graham Jenkin, Mark A Kirkland, Gopal Pande
Research output: Contribution to journal › Article › Other › peer-review
Umbilical cord blood (UCB) is one of the richest sources for hematopoietic stem/progenitor cells (HSPCs), with more than 3000 transplantations performed each year for the treatment of leukemia and other bone marrow, immunological, and hereditary diseases. However, transplantation of single cord blood units is mostly restricted to children, due to the limited number of HSPC per unit. This unit develops a method to increase the number of HSPCs in laboratory conditions by using cell-freematrices from bone marrowcells that mimic ‘human-body-niche-like’ conditions as biological scaffolds to support the ex vivo expansion of HSPCs. In this unit, we describe protocols for the isolation and characterization of HSPCs from UCB and their serum-free expansion on decellularized matrices. This method may also help to provide understanding of the biochemical organization of hematopoietic niches and lead to suggestions regarding the design of tissue engineering-based biomimetic scaffolds for HSPC expansion for clinical applications.
Original language | English |
---|---|
Pages (from-to) | 1C.15.1-1C.15.16 |
Number of pages | 16 |
Journal | Current Protocols in Stem Cell Biology |
Volume | 36 |
DOIs | |
Publication status | Published - 1 Feb 2016 |
Research output: Contribution to journal › Article › Research › peer-review