Expansion of human hematopoietic stem/progenitor cells on decellularized matrix scaffolds

Abhilasha Tiwari, Melinda L Tursky, Lakshmi Prasanna Nekkanti, Graham Jenkin, Mark A Kirkland, Gopal Pande

Research output: Contribution to journalArticleOtherpeer-review

4 Citations (Scopus)

Abstract

Umbilical cord blood (UCB) is one of the richest sources for hematopoietic stem/progenitor cells (HSPCs), with more than 3000 transplantations performed each year for the treatment of leukemia and other bone marrow, immunological, and hereditary diseases. However, transplantation of single cord blood units is mostly restricted to children, due to the limited number of HSPC per unit. This unit develops a method to increase the number of HSPCs in laboratory conditions by using cell-freematrices from bone marrowcells that mimic ‘human-body-niche-like’ conditions as biological scaffolds to support the ex vivo expansion of HSPCs. In this unit, we describe protocols for the isolation and characterization of HSPCs from UCB and their serum-free expansion on decellularized matrices. This method may also help to provide understanding of the biochemical organization of hematopoietic niches and lead to suggestions regarding the design of tissue engineering-based biomimetic scaffolds for HSPC expansion for clinical applications.

Original languageEnglish
Pages (from-to)1C.15.1-1C.15.16
Number of pages16
JournalCurrent Protocols in Stem Cell Biology
Volume36
DOIs
Publication statusPublished - 1 Feb 2016

Keywords

  • hematopoietic niche
  • decellularized biological scaffold
  • extracellular matrix
  • umbilical cord blood
  • caffold extracellular matrix umbilical cord blood stem

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