Projects per year
Abstract
Aromatic nitration reactions are a cornerstone of organic chemistry, but are challenging to scale due to corrosive reagents and elevated temperatures. The cytochrome P450 TxtE nitrates the indole 4-position of l-tryptophan at room temperature using NO, O2 and NADPH, and has potential to be developed into a useful aromatic nitration biocatalyst. However, its narrow substrate scope (requiring both the α-amino acid and indole functionalities) have hindered this. Screening of an R59 mutant library of a TxtE-reductase fusion protein identified a variant (R59C) that nitrates tryptamine, which is not accepted by native TxtE. This variant exhibits a broader substrate scope than the wild type enzyme and is able to nitrate a range of tryptamine analogues, with significant alterations to the aromatic and aminoethyl moieties.
Original language | English |
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Pages (from-to) | 2262-2265 |
Number of pages | 4 |
Journal | ChemBioChem |
Volume | 22 |
Issue number | 13 |
DOIs | |
Publication status | Published - 1 Jul 2021 |
Keywords
- Biocatalysis
- Indole
- Nitration
- Protein Engineering
Projects
- 1 Active
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ARC Centre of Excellence for Innovations in Peptide and Protein Science
Craik, D. J., Payne, R., Belov, K., Jolliffe, K. A., New, E., Fairlie, D., King, G. F., Henriques, S. T., Otting, G., Malins, L., Jackson, C., Cryle, M., Challis, G., Colgrave, M., Ploegh, H., Baker, D. A., Suga, H., Davis, B. G., Pentelute, B., van der Donk, W., Tavassoli, A. & Savage, G.
23/12/20 → 21/01/28
Project: Research