Exosome trapping and enrichment using a sound wave activated nano-sieve (SWANS)

Ruhollah Habibi, Vincent He, Sara Ghavamian, Alex De Marco, Tzong Hsien Lee, Marie Isabel Aguilar, Dandan Zhu, Rebecca Lim, Adrian Neild

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Abstract

Exosomes, a form of extracellular vesicle, are an important precursor in regenerative medicine. Microfluidic methods exist to capture these sub-micrometer sized objects from small quantities of sample, ideal for multiple diagnostic applications. To address the challenge of extraction from large volumes, we use the visual access offered by microfluidic techniques to probe the physical mechanisms behind a method which is compatible with future upscaling. The sound wave actuated nano-sieve uses resonant modes in a packed bed of microparticles to exert trapping forces on nanoparticles. Here, we examine the role of the microparticle size, demonstrating better performance from 15 μm particles than 7 μm particles. When applied to biological samples, we demonstrate for the first time that a packed bed of these larger particles is capable of capturing exosomes and liposomes, the captured particles being on average 20 to 40 times smaller than the pores within the trapped bed. This journal is

Original languageEnglish
Pages (from-to)3633-3643
Number of pages11
JournalLab on a Chip
Volume20
Issue number19
DOIs
Publication statusPublished - 28 Aug 2020

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