Exogenous Mtv-7 superantigen transgene expression in major histocompatibility complex class II I-E- mice reconstituted with embryonic stem cell-derived hematopoietic stem cells

Cynthia A. Chambers, Joonsoo Kang, Judy Pawling, Brigitte Huber, Nobumichi Hozumi, Andras Nagy

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

Direct genetic manipulation of hematopoietic cells is limited by the lack of an established hematopoietic stem cell line. It has been demonstrated that embryonic stem (ES) cell mutually implies tetraploid embryos are completely ES cell-derived and that fetal liver (FL) cells from these embryos support hematopoiesis in lethally irradiated recipients. In this report, we demonstrate that FL cells from ES cell mutually implies tetraploid embryos support normal lymphopoiesis and T-cell repertoire development. Moreover, the introduction of the Mtv-7 superantigen transgene coding for minor lymphocyte stimulatory antigen I into murine hematopoietic cells via reconstitution with ES cell mutually implies tetraploid FL cells demonstrates that this method can effectively confer stable genetic changes into the hematopoietic tissues without going through the germ line. Long-term and secondary reconstitution with ES cell mutually implies tetraploid FL cells expressing the Mtv-7 superantigen transgene clonally deleted minor lymphocyte stimulatory antigen 1-reactive T-cell receptor V(β)6+, -8.1+, and -9+ T cells, but not V(β)7+ T cells, in H-2(b) (I-E-) mice. This model system will be extremely important for analyzing structure-function relationships of molecules involved in proliferation, differentiation, and selection of hematopoietic cells in vivo and for examining hematopoiesis-specific effects of mutations that are lethal during embryogenesis.

Original languageEnglish
Pages (from-to)1138-1142
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number3
DOIs
Publication statusPublished - 1 Feb 1994
Externally publishedYes

Keywords

  • minor lymphocyte stimulatory antigen I

Cite this