Examination of the interaction between a membrane active peptide and artificial bilayers by dual polarisation interferometry

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Abstract

Examining the interaction of peptides with lipid bilayers to determine binding kinetics is often performed using surface plasmon resonance (SPR). Here we describe the technique of dual polarisation interferometry (DPI) that provides not only information on the kinetics of the peptide binding to the bilayer,but also how the peptide affects the lipid order of the bilayer.
Original languageEnglish
Article numbere2087
Number of pages12
JournalBio-protocol
Volume7
Issue number1
DOIs
Publication statusPublished - 5 Jan 2017

Keywords

  • membrane interaction
  • bilayer
  • peptide
  • kinetics
  • lipid order
  • protein-lipid interaction

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