Ex vivo dual perfusion of the human placenta: Disease simulation, therapeutic pharmacokinetics and analysis of off-target effects

Paul Brownbill, Neil Sebire, Erin V. McGillick, Stacey Ellery, Padma Murthi

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

6 Citations (Scopus)

Abstract

In recent years ex vivo dual perfusion of the human placental lobule is seeing an international renaissance in its application to understanding fetal health and development. Here, we discuss the methods and uses of this technique in the evaluation of (1) vascular function, (2) transplacental clearance, (3) hemodynamic and oxygenation changes associated with pregnancy complications on placental structure and function, and (4) placental toxicology and post-perfusion evaluation of tissue architecture.

Original languageEnglish
Title of host publicationPreeclampsia
Subtitle of host publicationMethods and Protocols
EditorsPadma Murthi, Cathy Vaillancourt
Place of PublicationNY USA
PublisherHumana Press
Pages173-189
Number of pages17
Volume1710
ISBN (Electronic)978-1-4939-7498-6
ISBN (Print)978-1-4939-7497-9
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1710
ISSN (Print)1064-3745

Keywords

  • Fetoplacental
  • Methods
  • Off-target effects
  • Perfusion
  • Pharmacokinetics
  • Placenta
  • Preeclampsia
  • Structural integrity
  • Vascular resistance

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