Evolutionary dynamics of Clostridium difficile over short and long time scales

Miao He; Mohammed Sebaihia; Trevor D. Lawley; Richard A. Stabler; Lisa F. Dawson; Melissa J. Martin; Kathryn E. Holt; Helena M.B. Seth-Smith; Michael A. Quail; Richard Rance; Karen Brooks; Carol Churcher; David Harris; Stephen D. Bentley; Christine Burrows; Louise Clark; Craig Corton; Vicky Murray; Graham Rose; Scott Thurston; Andries Van Tonder; Danielle Walker; Brendan W. Wren; Gordon Dougan; Julian Parkhill

doi:10.1073/pnas.0914322107

Evolutionary dynamics of Clostridium difficile over short and long time scales

Miao He, Mohammed Sebaihia, Trevor D. Lawley, Richard A. Stabler, Lisa F. Dawson, Melissa J. Martin, Kathryn E. Holt, Helena M.B. Seth-Smith, Michael A. Quail, Richard Rance, Karen Brooks, Carol Churcher, David Harris, Stephen D. Bentley, Christine Burrows, Louise Clark, Craig Corton, Vicky Murray, Graham Rose, Scott ThurstonAndries Van Tonder, Danielle Walker, Brendan W. Wren, Gordon Dougan, Julian Parkhill

Microbiology

Research output: Contribution to journal › Article › Research › peer-review

295 Citations (Scopus)

Abstract

Clostridium difficile has rapidly emerged as the leading cause of antibiotic-associated diarrheal disease, with the transcontinental spread of various PCR ribotypes, including 001, 017, 027 and 078. However, the genetic basis for the emergence of C. difficile as a human pathogen is unclear. Whole genome sequencing was used to analyze genetic variation and virulence of a diverse collection of thirty C. difficile isolates, to determine both macro and microevolution of the species. Horizontal gene transfer and large-scale recombination of core genes has shaped the C. difficile genome over both short and long time scales. Phylogenetic analysis demonstrates C. difficile is a genetically diverse species, which has evolved within the last 1.1-85 million years. By contrast, the disease-causing isolates have arisen from multiple lineages, suggesting that virulence evolved independently in the highly epidemic lineages.

Original language	English
Pages (from-to)	7527-7532
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	107
Issue number	16
DOIs	https://doi.org/10.1073/pnas.0914322107
Publication status	Published - 20 Apr 2010

Keywords

Homologous recombination
Horizontal gene transfer
Single nucleotide polymorphism

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1073/pnas.0914322107

Cite this

He, M., Sebaihia, M., Lawley, T. D., Stabler, R. A., Dawson, L. F., Martin, M. J., Holt, K. E., Seth-Smith, H. M. B., Quail, M. A., Rance, R., Brooks, K., Churcher, C., Harris, D., Bentley, S. D., Burrows, C., Clark, L., Corton, C., Murray, V., Rose, G., ... Parkhill, J. (2010). Evolutionary dynamics of Clostridium difficile over short and long time scales. Proceedings of the National Academy of Sciences of the United States of America, 107(16), 7527-7532. https://doi.org/10.1073/pnas.0914322107

@article{93e0d4203fe54145b937175d781ca9e2,

title = "Evolutionary dynamics of Clostridium difficile over short and long time scales",

abstract = "Clostridium difficile has rapidly emerged as the leading cause of antibiotic-associated diarrheal disease, with the transcontinental spread of various PCR ribotypes, including 001, 017, 027 and 078. However, the genetic basis for the emergence of C. difficile as a human pathogen is unclear. Whole genome sequencing was used to analyze genetic variation and virulence of a diverse collection of thirty C. difficile isolates, to determine both macro and microevolution of the species. Horizontal gene transfer and large-scale recombination of core genes has shaped the C. difficile genome over both short and long time scales. Phylogenetic analysis demonstrates C. difficile is a genetically diverse species, which has evolved within the last 1.1-85 million years. By contrast, the disease-causing isolates have arisen from multiple lineages, suggesting that virulence evolved independently in the highly epidemic lineages.",

keywords = "Homologous recombination, Horizontal gene transfer, Single nucleotide polymorphism",

author = "Miao He and Mohammed Sebaihia and Lawley, {Trevor D.} and Stabler, {Richard A.} and Dawson, {Lisa F.} and Martin, {Melissa J.} and Holt, {Kathryn E.} and Seth-Smith, {Helena M.B.} and Quail, {Michael A.} and Richard Rance and Karen Brooks and Carol Churcher and David Harris and Bentley, {Stephen D.} and Christine Burrows and Louise Clark and Craig Corton and Vicky Murray and Graham Rose and Scott Thurston and {Van Tonder}, Andries and Danielle Walker and Wren, {Brendan W.} and Gordon Dougan and Julian Parkhill",

year = "2010",

month = apr,

day = "20",

doi = "10.1073/pnas.0914322107",

language = "English",

volume = "107",

pages = "7527--7532",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "16",

}

He, M, Sebaihia, M, Lawley, TD, Stabler, RA, Dawson, LF, Martin, MJ, Holt, KE, Seth-Smith, HMB, Quail, MA, Rance, R, Brooks, K, Churcher, C, Harris, D, Bentley, SD, Burrows, C, Clark, L, Corton, C, Murray, V, Rose, G, Thurston, S, Van Tonder, A, Walker, D, Wren, BW, Dougan, G & Parkhill, J 2010, 'Evolutionary dynamics of Clostridium difficile over short and long time scales', Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 16, pp. 7527-7532. https://doi.org/10.1073/pnas.0914322107

TY - JOUR

T1 - Evolutionary dynamics of Clostridium difficile over short and long time scales

AU - He, Miao

AU - Sebaihia, Mohammed

AU - Lawley, Trevor D.

AU - Stabler, Richard A.

AU - Dawson, Lisa F.

AU - Martin, Melissa J.

AU - Holt, Kathryn E.

AU - Seth-Smith, Helena M.B.

AU - Quail, Michael A.

AU - Rance, Richard

AU - Brooks, Karen

AU - Churcher, Carol

AU - Harris, David

AU - Bentley, Stephen D.

AU - Burrows, Christine

AU - Clark, Louise

AU - Corton, Craig

AU - Murray, Vicky

AU - Rose, Graham

AU - Thurston, Scott

AU - Van Tonder, Andries

AU - Walker, Danielle

AU - Wren, Brendan W.

AU - Dougan, Gordon

AU - Parkhill, Julian

PY - 2010/4/20

Y1 - 2010/4/20

N2 - Clostridium difficile has rapidly emerged as the leading cause of antibiotic-associated diarrheal disease, with the transcontinental spread of various PCR ribotypes, including 001, 017, 027 and 078. However, the genetic basis for the emergence of C. difficile as a human pathogen is unclear. Whole genome sequencing was used to analyze genetic variation and virulence of a diverse collection of thirty C. difficile isolates, to determine both macro and microevolution of the species. Horizontal gene transfer and large-scale recombination of core genes has shaped the C. difficile genome over both short and long time scales. Phylogenetic analysis demonstrates C. difficile is a genetically diverse species, which has evolved within the last 1.1-85 million years. By contrast, the disease-causing isolates have arisen from multiple lineages, suggesting that virulence evolved independently in the highly epidemic lineages.

AB - Clostridium difficile has rapidly emerged as the leading cause of antibiotic-associated diarrheal disease, with the transcontinental spread of various PCR ribotypes, including 001, 017, 027 and 078. However, the genetic basis for the emergence of C. difficile as a human pathogen is unclear. Whole genome sequencing was used to analyze genetic variation and virulence of a diverse collection of thirty C. difficile isolates, to determine both macro and microevolution of the species. Horizontal gene transfer and large-scale recombination of core genes has shaped the C. difficile genome over both short and long time scales. Phylogenetic analysis demonstrates C. difficile is a genetically diverse species, which has evolved within the last 1.1-85 million years. By contrast, the disease-causing isolates have arisen from multiple lineages, suggesting that virulence evolved independently in the highly epidemic lineages.

KW - Homologous recombination

KW - Horizontal gene transfer

KW - Single nucleotide polymorphism

UR - http://www.scopus.com/inward/record.url?scp=77952168929&partnerID=8YFLogxK

U2 - 10.1073/pnas.0914322107

DO - 10.1073/pnas.0914322107

M3 - Article

C2 - 20368420

AN - SCOPUS:77952168929

SN - 0027-8424

VL - 107

SP - 7527

EP - 7532

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 16

ER -

Evolutionary dynamics of Clostridium difficile over short and long time scales

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Cite this