TY - JOUR
T1 - Evolution of B cell responses to Clec9A-targeted antigen
AU - Park, Hae-Young
AU - Light, Amanda
AU - Lahoud, Mireille
AU - Caminschi, Irina
AU - Tarlinton, David M
AU - Shortman, Ken
PY - 2013
Y1 - 2013
N2 - The response of B cells to Ag targeted to Clec9A on dendritic cells was followed using the hapten nitrophenol (NP) conjugated to rat Ig carrier. Injection of small amounts of NP conjugated to anti-Clec9A in the absence of adjuvants gave high and very prolonged Ab responses, approaching those obtained by high doses of nontargeted NP-protein conjugates with alum adjuvant. The response to NP-anti-Clec9A included the transient formation of germinal centers, maturation of Ab affinity, and some memory B cell formation. Serum Ab titers remained high 35 wk postimmunization, well after the initial follicular response had faded. The results suggest Clec9A-targeting strategies for improving Ab responses to vaccine Ags. ? 2013 by The American Association of Immunologists, Inc.
AB - The response of B cells to Ag targeted to Clec9A on dendritic cells was followed using the hapten nitrophenol (NP) conjugated to rat Ig carrier. Injection of small amounts of NP conjugated to anti-Clec9A in the absence of adjuvants gave high and very prolonged Ab responses, approaching those obtained by high doses of nontargeted NP-protein conjugates with alum adjuvant. The response to NP-anti-Clec9A included the transient formation of germinal centers, maturation of Ab affinity, and some memory B cell formation. Serum Ab titers remained high 35 wk postimmunization, well after the initial follicular response had faded. The results suggest Clec9A-targeting strategies for improving Ab responses to vaccine Ags. ? 2013 by The American Association of Immunologists, Inc.
UR - http://www.jimmunol.org/content/191/10/4919.full.pdf+html
U2 - 10.4049/jimmunol.1301947
DO - 10.4049/jimmunol.1301947
M3 - Article
SN - 0022-1767
VL - 191
SP - 4919
EP - 4925
JO - Journal of Immunology
JF - Journal of Immunology
IS - 10
ER -
