Evolution of B cell responses to Clec9A-targeted antigen

Hae-Young Park, Amanda Light, Mireille Lahoud, Irina Caminschi, David M Tarlinton, Ken Shortman

Research output: Contribution to journalArticleResearchpeer-review

24 Citations (Scopus)

Abstract

The response of B cells to Ag targeted to Clec9A on dendritic cells was followed using the hapten nitrophenol (NP) conjugated to rat Ig carrier. Injection of small amounts of NP conjugated to anti-Clec9A in the absence of adjuvants gave high and very prolonged Ab responses, approaching those obtained by high doses of nontargeted NP-protein conjugates with alum adjuvant. The response to NP-anti-Clec9A included the transient formation of germinal centers, maturation of Ab affinity, and some memory B cell formation. Serum Ab titers remained high 35 wk postimmunization, well after the initial follicular response had faded. The results suggest Clec9A-targeting strategies for improving Ab responses to vaccine Ags. ? 2013 by The American Association of Immunologists, Inc.
Original languageEnglish
Pages (from-to)4919 - 4925
Number of pages7
JournalJournal of Immunology
Volume191
Issue number10
DOIs
Publication statusPublished - 2013

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