Evidence that the greening ligand in native butyryl-CoA dehydrogenase is a CoA persulfide.

G. Williamson, P. C. Engel, J. P. Mizzer, C. Thorpe, V. Massey

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63 Citations (Scopus)

Abstract

Yellow butyryl-CoA dehydrogenase and general acyl-CoA dehydrogenase are "greened" by a mixture of coenzyme A plus elemental sulfur. The resultant stable complex contains an identical ligand with that present in native green butyryl-CoA dehydrogenase and has the same broad absorption band centered at 710 nm. Evidence is presented that the greening ligand is a CoA persulfide, possibly a mimic of the substrate carbanion thought to be generated early in the normal catalytic cycle. Variation in the position of the long wavelength band on replacement of FAD by a series of analogs of differing oxidation-reduction potential is consistent with a charge-transfer complex between a persulfide as the donor and oxidized flavin as the acceptor. The possible physiological and metabolic significance is discussed.

Original languageEnglish
Pages (from-to)4314-4320
Number of pages7
JournalJournal of Biological Chemistry
Volume257
Issue number8
Publication statusPublished - 25 Apr 1982
Externally publishedYes

Cite this

Williamson, G. ; Engel, P. C. ; Mizzer, J. P. ; Thorpe, C. ; Massey, V. / Evidence that the greening ligand in native butyryl-CoA dehydrogenase is a CoA persulfide. In: Journal of Biological Chemistry. 1982 ; Vol. 257, No. 8. pp. 4314-4320.
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Williamson, G, Engel, PC, Mizzer, JP, Thorpe, C & Massey, V 1982, 'Evidence that the greening ligand in native butyryl-CoA dehydrogenase is a CoA persulfide.', Journal of Biological Chemistry, vol. 257, no. 8, pp. 4314-4320.

Evidence that the greening ligand in native butyryl-CoA dehydrogenase is a CoA persulfide. / Williamson, G.; Engel, P. C.; Mizzer, J. P.; Thorpe, C.; Massey, V.

In: Journal of Biological Chemistry, Vol. 257, No. 8, 25.04.1982, p. 4314-4320.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Evidence that the greening ligand in native butyryl-CoA dehydrogenase is a CoA persulfide.

AU - Williamson, G.

AU - Engel, P. C.

AU - Mizzer, J. P.

AU - Thorpe, C.

AU - Massey, V.

PY - 1982/4/25

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