Evidence of prokineticin dysregulation in fallopian tube from women with ectopic pregnancy

Julie L.V. Shaw, Fiona C. Denison, Jemma Evans, Kimberley Durno, Alistair R. Williams, Gary Entrican, Hilary O.D. Critchley, Henry N. Jabbour, Andrew W. Horne

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Objective: To demonstrate expression and regulation of prokineticins (PROKs) and their receptors (PROKRs) in fallopian tube (FT) from women who are not pregnant and women with ectopic pregnancy (EP). Design: Tissue analysis. Setting: Large United Kingdom teaching hospital. Patient(s): Women undergoing hysterectomy for benign gynecological conditions (n = 15) and surgery for EP (n = 16). Intervention(s): Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry were used to determine FT PROK/PROKR messenger RNA (mRNA) expression and protein localization, respectively. The PROK/PROKR levels were measured in tubal explant cultures stimulated with estrogen (E) and progestogen. Main Outcome Measure(s): Differential expression of PROK and PROKR. Result(s): The FT PROK2 and PROKR1 mRNA levels were up-regulated during the P-dominant midluteal phase of the menstrual cycle. Increased PROKR1 expression was observed in tubal explant cultures treated with medroxy-progesterone acetate (MPA). The PROK and PROKR proteins were localized to the epithelium and smooth muscle layers of the FT. The PROKR1 and PROKR2 mRNA levels were lower in FT from women with EP compared with nonpregnant FT from the midluteal phase. Conclusion(s): These data suggest a potential role for PROKs in FT function. The PROKs are known to affect smooth muscle contraction in the gut. Dysregulated PROK expression in FT could affect FT smooth muscle contractility and embryo-tubal transport, providing a potential cause for EP.

Original languageEnglish
JournalFertility and Sterility
Volume94
Issue number5
DOIs
Publication statusPublished - 1 Oct 2010

Keywords

  • ectopic pregnancy
  • Fallopian tube
  • menstrual cycle
  • prokineticin
  • smooth muscle contractility

Cite this

Shaw, Julie L.V. ; Denison, Fiona C. ; Evans, Jemma ; Durno, Kimberley ; Williams, Alistair R. ; Entrican, Gary ; Critchley, Hilary O.D. ; Jabbour, Henry N. ; Horne, Andrew W. / Evidence of prokineticin dysregulation in fallopian tube from women with ectopic pregnancy. In: Fertility and Sterility. 2010 ; Vol. 94, No. 5.
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title = "Evidence of prokineticin dysregulation in fallopian tube from women with ectopic pregnancy",
abstract = "Objective: To demonstrate expression and regulation of prokineticins (PROKs) and their receptors (PROKRs) in fallopian tube (FT) from women who are not pregnant and women with ectopic pregnancy (EP). Design: Tissue analysis. Setting: Large United Kingdom teaching hospital. Patient(s): Women undergoing hysterectomy for benign gynecological conditions (n = 15) and surgery for EP (n = 16). Intervention(s): Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry were used to determine FT PROK/PROKR messenger RNA (mRNA) expression and protein localization, respectively. The PROK/PROKR levels were measured in tubal explant cultures stimulated with estrogen (E) and progestogen. Main Outcome Measure(s): Differential expression of PROK and PROKR. Result(s): The FT PROK2 and PROKR1 mRNA levels were up-regulated during the P-dominant midluteal phase of the menstrual cycle. Increased PROKR1 expression was observed in tubal explant cultures treated with medroxy-progesterone acetate (MPA). The PROK and PROKR proteins were localized to the epithelium and smooth muscle layers of the FT. The PROKR1 and PROKR2 mRNA levels were lower in FT from women with EP compared with nonpregnant FT from the midluteal phase. Conclusion(s): These data suggest a potential role for PROKs in FT function. The PROKs are known to affect smooth muscle contraction in the gut. Dysregulated PROK expression in FT could affect FT smooth muscle contractility and embryo-tubal transport, providing a potential cause for EP.",
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Shaw, JLV, Denison, FC, Evans, J, Durno, K, Williams, AR, Entrican, G, Critchley, HOD, Jabbour, HN & Horne, AW 2010, 'Evidence of prokineticin dysregulation in fallopian tube from women with ectopic pregnancy' Fertility and Sterility, vol. 94, no. 5. https://doi.org/10.1016/j.fertnstert.2009.10.061

Evidence of prokineticin dysregulation in fallopian tube from women with ectopic pregnancy. / Shaw, Julie L.V.; Denison, Fiona C.; Evans, Jemma; Durno, Kimberley; Williams, Alistair R.; Entrican, Gary; Critchley, Hilary O.D.; Jabbour, Henry N.; Horne, Andrew W.

In: Fertility and Sterility, Vol. 94, No. 5, 01.10.2010.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Evidence of prokineticin dysregulation in fallopian tube from women with ectopic pregnancy

AU - Shaw, Julie L.V.

AU - Denison, Fiona C.

AU - Evans, Jemma

AU - Durno, Kimberley

AU - Williams, Alistair R.

AU - Entrican, Gary

AU - Critchley, Hilary O.D.

AU - Jabbour, Henry N.

AU - Horne, Andrew W.

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KW - Fallopian tube

KW - menstrual cycle

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