Evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974

Kelly L. Wyres, Andries van Tonder, Lotte M. Lambertsen, Regine Hakenbeck, Julian Parkhill, Stephen D. Bentley, Angela B. Brueggemann

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background: Antimicrobial resistance among pneumococci has greatly increased over the past two to three decades. Resistance to tetracycline (tet(M)), chloramphenicol (cat) and macrolides (erm(B) and/or mef(A/E)) is generally conferred by acquisition of specific genes that are associated with mobile genetic elements, including those of the Tn916 and Tn5252 families. The first tetracycline-, chloramphenicol- and macrolide-resistant pneumococci were detected between 1962 and 1970; however, until now the oldest pneumococcus shown to harbour Tn916 and/or Tn5252 was isolated in 1974. In this study the genomes of 38 pneumococci isolated prior to 1974 were probed for the presence of tet(M), cat, erm(B), mef(A/E) and int (integrase) to indicate the presence of Tn916/Tn5252-like elements.Results: Two Tn916-like, tet(M)-containing, elements were identified among pneumococci dated 1967 and 1968. The former element was highly similar to that of the PMEN1 multidrug-resistant, globally-distributed pneumococcal reference strain, which was isolated in 1984. The latter element was associated with a streptococcal phage. A third, novel genetic element, designated ICESpPN1, was identified in the genome of an isolate dated 1972. ICESpPN1 contained a region of similarity to Tn5252, a region of similarity to a pneumococcal pathogenicity island and novel lantibiotic synthesis/export-associated genes.Conclusions: These data confirm the existence of pneumococcal Tn916 elements in the first decade within which pneumococcal tetracycline resistance was described. Furthermore, the discovery of ICESpPN1 demonstrates the dynamic variability of pneumococcal genetic elements and is contrasted with the evidence for Tn916 stability.

Original languageEnglish
Article number500
JournalBMC Genomics
Volume14
Issue number1
DOIs
Publication statusPublished - 24 Jul 2013

Keywords

  • Antimicrobial resistance
  • ICE elements
  • Mobile genetic elements
  • Streptococcus pneumoniae
  • Tetracycline resistance

Cite this

Wyres, K. L., van Tonder, A., Lambertsen, L. M., Hakenbeck, R., Parkhill, J., Bentley, S. D., & Brueggemann, A. B. (2013). Evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974. BMC Genomics, 14(1), [500]. https://doi.org/10.1186/1471-2164-14-500
Wyres, Kelly L. ; van Tonder, Andries ; Lambertsen, Lotte M. ; Hakenbeck, Regine ; Parkhill, Julian ; Bentley, Stephen D. ; Brueggemann, Angela B. / Evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974. In: BMC Genomics. 2013 ; Vol. 14, No. 1.
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abstract = "Background: Antimicrobial resistance among pneumococci has greatly increased over the past two to three decades. Resistance to tetracycline (tet(M)), chloramphenicol (cat) and macrolides (erm(B) and/or mef(A/E)) is generally conferred by acquisition of specific genes that are associated with mobile genetic elements, including those of the Tn916 and Tn5252 families. The first tetracycline-, chloramphenicol- and macrolide-resistant pneumococci were detected between 1962 and 1970; however, until now the oldest pneumococcus shown to harbour Tn916 and/or Tn5252 was isolated in 1974. In this study the genomes of 38 pneumococci isolated prior to 1974 were probed for the presence of tet(M), cat, erm(B), mef(A/E) and int (integrase) to indicate the presence of Tn916/Tn5252-like elements.Results: Two Tn916-like, tet(M)-containing, elements were identified among pneumococci dated 1967 and 1968. The former element was highly similar to that of the PMEN1 multidrug-resistant, globally-distributed pneumococcal reference strain, which was isolated in 1984. The latter element was associated with a streptococcal phage. A third, novel genetic element, designated ICESpPN1, was identified in the genome of an isolate dated 1972. ICESpPN1 contained a region of similarity to Tn5252, a region of similarity to a pneumococcal pathogenicity island and novel lantibiotic synthesis/export-associated genes.Conclusions: These data confirm the existence of pneumococcal Tn916 elements in the first decade within which pneumococcal tetracycline resistance was described. Furthermore, the discovery of ICESpPN1 demonstrates the dynamic variability of pneumococcal genetic elements and is contrasted with the evidence for Tn916 stability.",
keywords = "Antimicrobial resistance, ICE elements, Mobile genetic elements, Streptococcus pneumoniae, Tetracycline resistance",
author = "Wyres, {Kelly L.} and {van Tonder}, Andries and Lambertsen, {Lotte M.} and Regine Hakenbeck and Julian Parkhill and Bentley, {Stephen D.} and Brueggemann, {Angela B.}",
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Wyres, KL, van Tonder, A, Lambertsen, LM, Hakenbeck, R, Parkhill, J, Bentley, SD & Brueggemann, AB 2013, 'Evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974' BMC Genomics, vol. 14, no. 1, 500. https://doi.org/10.1186/1471-2164-14-500

Evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974. / Wyres, Kelly L.; van Tonder, Andries; Lambertsen, Lotte M.; Hakenbeck, Regine; Parkhill, Julian; Bentley, Stephen D.; Brueggemann, Angela B.

In: BMC Genomics, Vol. 14, No. 1, 500, 24.07.2013.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974

AU - Wyres, Kelly L.

AU - van Tonder, Andries

AU - Lambertsen, Lotte M.

AU - Hakenbeck, Regine

AU - Parkhill, Julian

AU - Bentley, Stephen D.

AU - Brueggemann, Angela B.

PY - 2013/7/24

Y1 - 2013/7/24

N2 - Background: Antimicrobial resistance among pneumococci has greatly increased over the past two to three decades. Resistance to tetracycline (tet(M)), chloramphenicol (cat) and macrolides (erm(B) and/or mef(A/E)) is generally conferred by acquisition of specific genes that are associated with mobile genetic elements, including those of the Tn916 and Tn5252 families. The first tetracycline-, chloramphenicol- and macrolide-resistant pneumococci were detected between 1962 and 1970; however, until now the oldest pneumococcus shown to harbour Tn916 and/or Tn5252 was isolated in 1974. In this study the genomes of 38 pneumococci isolated prior to 1974 were probed for the presence of tet(M), cat, erm(B), mef(A/E) and int (integrase) to indicate the presence of Tn916/Tn5252-like elements.Results: Two Tn916-like, tet(M)-containing, elements were identified among pneumococci dated 1967 and 1968. The former element was highly similar to that of the PMEN1 multidrug-resistant, globally-distributed pneumococcal reference strain, which was isolated in 1984. The latter element was associated with a streptococcal phage. A third, novel genetic element, designated ICESpPN1, was identified in the genome of an isolate dated 1972. ICESpPN1 contained a region of similarity to Tn5252, a region of similarity to a pneumococcal pathogenicity island and novel lantibiotic synthesis/export-associated genes.Conclusions: These data confirm the existence of pneumococcal Tn916 elements in the first decade within which pneumococcal tetracycline resistance was described. Furthermore, the discovery of ICESpPN1 demonstrates the dynamic variability of pneumococcal genetic elements and is contrasted with the evidence for Tn916 stability.

AB - Background: Antimicrobial resistance among pneumococci has greatly increased over the past two to three decades. Resistance to tetracycline (tet(M)), chloramphenicol (cat) and macrolides (erm(B) and/or mef(A/E)) is generally conferred by acquisition of specific genes that are associated with mobile genetic elements, including those of the Tn916 and Tn5252 families. The first tetracycline-, chloramphenicol- and macrolide-resistant pneumococci were detected between 1962 and 1970; however, until now the oldest pneumococcus shown to harbour Tn916 and/or Tn5252 was isolated in 1974. In this study the genomes of 38 pneumococci isolated prior to 1974 were probed for the presence of tet(M), cat, erm(B), mef(A/E) and int (integrase) to indicate the presence of Tn916/Tn5252-like elements.Results: Two Tn916-like, tet(M)-containing, elements were identified among pneumococci dated 1967 and 1968. The former element was highly similar to that of the PMEN1 multidrug-resistant, globally-distributed pneumococcal reference strain, which was isolated in 1984. The latter element was associated with a streptococcal phage. A third, novel genetic element, designated ICESpPN1, was identified in the genome of an isolate dated 1972. ICESpPN1 contained a region of similarity to Tn5252, a region of similarity to a pneumococcal pathogenicity island and novel lantibiotic synthesis/export-associated genes.Conclusions: These data confirm the existence of pneumococcal Tn916 elements in the first decade within which pneumococcal tetracycline resistance was described. Furthermore, the discovery of ICESpPN1 demonstrates the dynamic variability of pneumococcal genetic elements and is contrasted with the evidence for Tn916 stability.

KW - Antimicrobial resistance

KW - ICE elements

KW - Mobile genetic elements

KW - Streptococcus pneumoniae

KW - Tetracycline resistance

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U2 - 10.1186/1471-2164-14-500

DO - 10.1186/1471-2164-14-500

M3 - Article

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