Evidence from binding studies for α2-adrenoceptors directly associated with glomeruli from rat kidney

Grant A. McPherson, Roger J. Summers

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Abstract

Binding of the α2-adrenoceptor radioligands [3H]clonidine and [3H]rauwolscine but not the α1-adrenoceptor radioligand [3H]prazosin was enhanced in membranes prepared from rat isolated renal glomeruli. [3H]Rauwolscine binding to glomeruli was stereoselective with respect to the (-)-isomer of noradrenaline and the order of potency of a series of antagonists for displacement of binding indicated that the α2-adrenoceptors in this preparation differ somewhat from those in some other species and tissues. Chemical sympathectomy produced no significant change in the number of sites labelled by [3H]rauwolscine indicating that few of the α2-adrenoceptors in glomerular membranes are associated with sympathetic nerve terminals.

Original languageEnglish
Pages (from-to)333-341
Number of pages9
JournalEuropean Journal of Pharmacology
Volume90
Issue number4
DOIs
Publication statusPublished - 17 Jun 1983

Keywords

  • Radioligand binding
  • Rat kidney
  • α-Adrenoceptors

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