Evidence for the localization of the Arabidopsis cytokinin receptors AHK3 and AHK4 in the endoplasmic reticulum

Katharina Caesar, Antje M K Thamm, Janika Witthoft, Kirstin Elgass, Peter Huppenberger, Christopher Grefen, Jakub Horak, Klaus Harter

Research output: Contribution to journalArticleResearchpeer-review

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Abstract

Cytokinins are hormones that are involved in various processes of plant growth and development. The model of cytokinin signalling starts with hormone perception through membrane-localized histidine kinase receptors. Although the biochemical properties and functions of these receptors have been extensively studied, there is no solid proof of their subcellular localization. Here, cell biological and biochemical evidence for the localization of functional fluorophor-tagged fusions of Arabidopsis histidine kinase 3 (AHK3) and 4 (AHK4), members of the cytokinin receptor family, in the endoplasmic reticulum (ER) is provided. Furthermore, membrane-bound AHK3 interacts with AHK4 in vivo. The ER localization and putative function of cytokinin receptors from the ER have major impacts on the concept of cytokinin perception and signalling, and hormonal cross-talk in plants.
Original languageEnglish
Pages (from-to)5571 - 5580
Number of pages10
JournalJournal of Experimental Botany
Volume62
Issue number15
DOIs
Publication statusPublished - 2011
Externally publishedYes

Cite this

Caesar, Katharina ; Thamm, Antje M K ; Witthoft, Janika ; Elgass, Kirstin ; Huppenberger, Peter ; Grefen, Christopher ; Horak, Jakub ; Harter, Klaus. / Evidence for the localization of the Arabidopsis cytokinin receptors AHK3 and AHK4 in the endoplasmic reticulum. In: Journal of Experimental Botany. 2011 ; Vol. 62, No. 15. pp. 5571 - 5580.
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title = "Evidence for the localization of the Arabidopsis cytokinin receptors AHK3 and AHK4 in the endoplasmic reticulum",
abstract = "Cytokinins are hormones that are involved in various processes of plant growth and development. The model of cytokinin signalling starts with hormone perception through membrane-localized histidine kinase receptors. Although the biochemical properties and functions of these receptors have been extensively studied, there is no solid proof of their subcellular localization. Here, cell biological and biochemical evidence for the localization of functional fluorophor-tagged fusions of Arabidopsis histidine kinase 3 (AHK3) and 4 (AHK4), members of the cytokinin receptor family, in the endoplasmic reticulum (ER) is provided. Furthermore, membrane-bound AHK3 interacts with AHK4 in vivo. The ER localization and putative function of cytokinin receptors from the ER have major impacts on the concept of cytokinin perception and signalling, and hormonal cross-talk in plants.",
author = "Katharina Caesar and Thamm, {Antje M K} and Janika Witthoft and Kirstin Elgass and Peter Huppenberger and Christopher Grefen and Jakub Horak and Klaus Harter",
year = "2011",
doi = "10.1093/jxb/err238",
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Caesar, K, Thamm, AMK, Witthoft, J, Elgass, K, Huppenberger, P, Grefen, C, Horak, J & Harter, K 2011, 'Evidence for the localization of the Arabidopsis cytokinin receptors AHK3 and AHK4 in the endoplasmic reticulum', Journal of Experimental Botany, vol. 62, no. 15, pp. 5571 - 5580. https://doi.org/10.1093/jxb/err238

Evidence for the localization of the Arabidopsis cytokinin receptors AHK3 and AHK4 in the endoplasmic reticulum. / Caesar, Katharina; Thamm, Antje M K; Witthoft, Janika; Elgass, Kirstin; Huppenberger, Peter; Grefen, Christopher; Horak, Jakub; Harter, Klaus.

In: Journal of Experimental Botany, Vol. 62, No. 15, 2011, p. 5571 - 5580.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Evidence for the localization of the Arabidopsis cytokinin receptors AHK3 and AHK4 in the endoplasmic reticulum

AU - Caesar, Katharina

AU - Thamm, Antje M K

AU - Witthoft, Janika

AU - Elgass, Kirstin

AU - Huppenberger, Peter

AU - Grefen, Christopher

AU - Horak, Jakub

AU - Harter, Klaus

PY - 2011

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N2 - Cytokinins are hormones that are involved in various processes of plant growth and development. The model of cytokinin signalling starts with hormone perception through membrane-localized histidine kinase receptors. Although the biochemical properties and functions of these receptors have been extensively studied, there is no solid proof of their subcellular localization. Here, cell biological and biochemical evidence for the localization of functional fluorophor-tagged fusions of Arabidopsis histidine kinase 3 (AHK3) and 4 (AHK4), members of the cytokinin receptor family, in the endoplasmic reticulum (ER) is provided. Furthermore, membrane-bound AHK3 interacts with AHK4 in vivo. The ER localization and putative function of cytokinin receptors from the ER have major impacts on the concept of cytokinin perception and signalling, and hormonal cross-talk in plants.

AB - Cytokinins are hormones that are involved in various processes of plant growth and development. The model of cytokinin signalling starts with hormone perception through membrane-localized histidine kinase receptors. Although the biochemical properties and functions of these receptors have been extensively studied, there is no solid proof of their subcellular localization. Here, cell biological and biochemical evidence for the localization of functional fluorophor-tagged fusions of Arabidopsis histidine kinase 3 (AHK3) and 4 (AHK4), members of the cytokinin receptor family, in the endoplasmic reticulum (ER) is provided. Furthermore, membrane-bound AHK3 interacts with AHK4 in vivo. The ER localization and putative function of cytokinin receptors from the ER have major impacts on the concept of cytokinin perception and signalling, and hormonal cross-talk in plants.

UR - http://www.ncbi.nlm.nih.gov/pubmed/21841169

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