Evidence for a lack of inositol - (1,4,5)trisphosphate kinase activity in norepinephrine-perfused rat hearts

Elizabeth A. Woodcock, A. Ian Smith, Catherine A. Wallace, L. Barbara Schmauk White

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19 Citations (Scopus)

Abstract

The products of the phosphatidylinositol turnover pathway in norepinephrine-perfused hearts have been examined using high performance liquid chromatography. Inositol-1 phosphate, inositol-(1,4)bisphosphate and inositol-(1,4,5)trisphosphate were all increased in response to norepinephrine stimulation. However, at perfusion times from 5 sec to 20 min there was no appearance of inositol-(1,3,4,5)tetrakisphosphate or inositol-(1,3,4)trisphosphate. This suggests the absence of the inositol-(1,4,5)trisphosphate phosphorylation/dephosphorylation pathway in heart and demonstrates that this secondary pathway is not essential to the functioning of the phosphatidylinositol turnover cycle.

Original languageEnglish
Pages (from-to)68-77
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume148
Issue number1
DOIs
Publication statusPublished - 14 Oct 1987

Cite this

Woodcock, Elizabeth A. ; Smith, A. Ian ; Wallace, Catherine A. ; White, L. Barbara Schmauk. / Evidence for a lack of inositol - (1,4,5)trisphosphate kinase activity in norepinephrine-perfused rat hearts. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 148, No. 1. pp. 68-77.
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Evidence for a lack of inositol - (1,4,5)trisphosphate kinase activity in norepinephrine-perfused rat hearts. / Woodcock, Elizabeth A.; Smith, A. Ian; Wallace, Catherine A.; White, L. Barbara Schmauk.

In: Biochemical and Biophysical Research Communications, Vol. 148, No. 1, 14.10.1987, p. 68-77.

Research output: Contribution to journalArticleResearchpeer-review

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