TY - JOUR
T1 - Evidence for a lack of inositol - (1,4,5)trisphosphate kinase activity in norepinephrine-perfused rat hearts
AU - Woodcock, Elizabeth A.
AU - Smith, A. Ian
AU - Wallace, Catherine A.
AU - White, L. Barbara Schmauk
PY - 1987/10/14
Y1 - 1987/10/14
N2 - The products of the phosphatidylinositol turnover pathway in norepinephrine-perfused hearts have been examined using high performance liquid chromatography. Inositol-1 phosphate, inositol-(1,4)bisphosphate and inositol-(1,4,5)trisphosphate were all increased in response to norepinephrine stimulation. However, at perfusion times from 5 sec to 20 min there was no appearance of inositol-(1,3,4,5)tetrakisphosphate or inositol-(1,3,4)trisphosphate. This suggests the absence of the inositol-(1,4,5)trisphosphate phosphorylation/dephosphorylation pathway in heart and demonstrates that this secondary pathway is not essential to the functioning of the phosphatidylinositol turnover cycle.
AB - The products of the phosphatidylinositol turnover pathway in norepinephrine-perfused hearts have been examined using high performance liquid chromatography. Inositol-1 phosphate, inositol-(1,4)bisphosphate and inositol-(1,4,5)trisphosphate were all increased in response to norepinephrine stimulation. However, at perfusion times from 5 sec to 20 min there was no appearance of inositol-(1,3,4,5)tetrakisphosphate or inositol-(1,3,4)trisphosphate. This suggests the absence of the inositol-(1,4,5)trisphosphate phosphorylation/dephosphorylation pathway in heart and demonstrates that this secondary pathway is not essential to the functioning of the phosphatidylinositol turnover cycle.
UR - http://www.scopus.com/inward/record.url?scp=0023627608&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(87)91077-1
DO - 10.1016/0006-291X(87)91077-1
M3 - Article
C2 - 2823816
AN - SCOPUS:0023627608
SN - 0006-291X
VL - 148
SP - 68
EP - 77
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -