Evidence for a lack of inositol - (1,4,5)trisphosphate kinase activity in norepinephrine-perfused rat hearts

Elizabeth A. Woodcock, A. Ian Smith, Catherine A. Wallace, L. Barbara Schmauk White

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The products of the phosphatidylinositol turnover pathway in norepinephrine-perfused hearts have been examined using high performance liquid chromatography. Inositol-1 phosphate, inositol-(1,4)bisphosphate and inositol-(1,4,5)trisphosphate were all increased in response to norepinephrine stimulation. However, at perfusion times from 5 sec to 20 min there was no appearance of inositol-(1,3,4,5)tetrakisphosphate or inositol-(1,3,4)trisphosphate. This suggests the absence of the inositol-(1,4,5)trisphosphate phosphorylation/dephosphorylation pathway in heart and demonstrates that this secondary pathway is not essential to the functioning of the phosphatidylinositol turnover cycle.

Original languageEnglish
Pages (from-to)68-77
Number of pages10
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - 14 Oct 1987

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