Evaluation of the role of galectins in parasite immunity

Sarah Preston, Jillian Dunphy, Travis Beddoe, Els Meeusen, Anna Young

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

14 Citations (Scopus)

Abstract

Galectin-11 and galectin-14 are ruminant galectins involved in parasitic infections. Although their roles in parasite immunity are still being elucidated, its appears that their functions are parasite specific. In gastrointestinal infections with the nematode Haemonchus contortus, both galectin-11 and galectin-14 appear to be protective. However, in a chronic infection of liver fluke, Fasciola hepatica, these galectins may aid parasite survival. This chapter discusses the methods designed to study parasitic infections in sheep, which have provided us with insight into the functions of galectin-11 and galectin-14 during host-parasite interactions. These methods include parasite cultivation and infection, galectin staining of host and parasite tissue, surface staining of parasites with recombinant galectins and in vitro assays to monitor the effect of galectins on larval development.
Original languageEnglish
Title of host publicationGalectins
Subtitle of host publicationMethods and Protocols
EditorsSean R Stowell, Richard D Cummings
Place of PublicationNew York NY USA
PublisherHumana Press
Pages371-395
Number of pages25
Edition1st
ISBN (Print)9781493913954
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1207
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Cite this