Evaluation of the Role of Bacterial Amyloid on Nucleoid Structure Using Cryo-Soft X-Ray Tomography

Antoine Cossa; Frank Wien; Florian Turbant; Tadeusz Kaczorowski; Grzegorz Węgrzyn; Véronique Arluison; Ana J. Pérez-Berná; Sylvain Trépout; Eva Pereiro

doi:10.1007/978-1-0716-2529-3_21

Evaluation of the Role of Bacterial Amyloid on Nucleoid Structure Using Cryo-Soft X-Ray Tomography

Antoine Cossa, Frank Wien, Florian Turbant, Tadeusz Kaczorowski, Grzegorz Węgrzyn, Véronique Arluison, Ana J. Pérez-Berná, Sylvain Trépout, Eva Pereiro

Cryo Electron Microscopy (CryoEM)

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

1 Citation (Scopus)

Abstract

Bacterial chromosomal DNA is packed within a non-membranous structure, the nucleoid, thanks to nucleoid associated proteins (NAPs). The role of bacterial amyloid has recently emerged among these NAPs, particularly with the nucleoid-associated protein Hfq that plays a direct role in DNA compaction. In this chapter, we present a 3D imaging technique, cryo-soft X-ray tomography (cryo-SXT) to obtain a detailed 3D visualization of subcellular bacterial structures, especially the nucleoid. Cryo-SXT imaging of native unlabeled cells enables observation of the nucleoid in 3D with a high resolution, allowing to evidence in vivo the role of amyloids on DNA compaction. The precise experimental methods to obtain 3D tomograms will be presented.

Original language	English
Title of host publication	Bacterial Amyloids
Subtitle of host publication	Methods and Protocols
Editors	Véronique Arluison, Frank Wien, Andrés Marcoleta
Place of Publication	New York NY USA
Publisher	Humana Press
Chapter	21
Pages	319-333
Number of pages	15
Edition	1st
ISBN (Electronic)	9781071625293
ISBN (Print)	9781071625286, 9781071625316
DOIs	https://doi.org/10.1007/978-1-0716-2529-3_21
Publication status	Published - 2022

Publication series

Name	Methods in Molecular Biology
Publisher	Springer
Volume	2538
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Bacterial amyloid
Cryo-tomography
Nucleoid
X-ray imaging

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10.1007/978-1-0716-2529-3_21

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Cossa, A., Wien, F., Turbant, F., Kaczorowski, T., Węgrzyn, G., Arluison, V., Pérez-Berná, A. J., Trépout, S., & Pereiro, E. (2022). Evaluation of the Role of Bacterial Amyloid on Nucleoid Structure Using Cryo-Soft X-Ray Tomography. In V. Arluison, F. Wien, & A. Marcoleta (Eds.), Bacterial Amyloids: Methods and Protocols (1st ed., pp. 319-333). (Methods in Molecular Biology; Vol. 2538). Humana Press. https://doi.org/10.1007/978-1-0716-2529-3_21

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abstract = "Bacterial chromosomal DNA is packed within a non-membranous structure, the nucleoid, thanks to nucleoid associated proteins (NAPs). The role of bacterial amyloid has recently emerged among these NAPs, particularly with the nucleoid-associated protein Hfq that plays a direct role in DNA compaction. In this chapter, we present a 3D imaging technique, cryo-soft X-ray tomography (cryo-SXT) to obtain a detailed 3D visualization of subcellular bacterial structures, especially the nucleoid. Cryo-SXT imaging of native unlabeled cells enables observation of the nucleoid in 3D with a high resolution, allowing to evidence in vivo the role of amyloids on DNA compaction. The precise experimental methods to obtain 3D tomograms will be presented.",

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Cossa, A, Wien, F, Turbant, F, Kaczorowski, T, Węgrzyn, G, Arluison, V, Pérez-Berná, AJ, Trépout, S & Pereiro, E 2022, Evaluation of the Role of Bacterial Amyloid on Nucleoid Structure Using Cryo-Soft X-Ray Tomography. in V Arluison, F Wien & A Marcoleta (eds), Bacterial Amyloids: Methods and Protocols. 1st edn, Methods in Molecular Biology, vol. 2538, Humana Press, New York NY USA, pp. 319-333. https://doi.org/10.1007/978-1-0716-2529-3_21

Evaluation of the Role of Bacterial Amyloid on Nucleoid Structure Using Cryo-Soft X-Ray Tomography. / Cossa, Antoine; Wien, Frank; Turbant, Florian et al.
Bacterial Amyloids: Methods and Protocols. ed. / Véronique Arluison; Frank Wien; Andrés Marcoleta. 1st. ed. New York NY USA: Humana Press, 2022. p. 319-333 (Methods in Molecular Biology; Vol. 2538).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

TY - CHAP

T1 - Evaluation of the Role of Bacterial Amyloid on Nucleoid Structure Using Cryo-Soft X-Ray Tomography

AU - Cossa, Antoine

AU - Wien, Frank

AU - Turbant, Florian

AU - Kaczorowski, Tadeusz

AU - Węgrzyn, Grzegorz

AU - Arluison, Véronique

AU - Pérez-Berná, Ana J.

AU - Trépout, Sylvain

AU - Pereiro, Eva

PY - 2022

Y1 - 2022

N2 - Bacterial chromosomal DNA is packed within a non-membranous structure, the nucleoid, thanks to nucleoid associated proteins (NAPs). The role of bacterial amyloid has recently emerged among these NAPs, particularly with the nucleoid-associated protein Hfq that plays a direct role in DNA compaction. In this chapter, we present a 3D imaging technique, cryo-soft X-ray tomography (cryo-SXT) to obtain a detailed 3D visualization of subcellular bacterial structures, especially the nucleoid. Cryo-SXT imaging of native unlabeled cells enables observation of the nucleoid in 3D with a high resolution, allowing to evidence in vivo the role of amyloids on DNA compaction. The precise experimental methods to obtain 3D tomograms will be presented.

AB - Bacterial chromosomal DNA is packed within a non-membranous structure, the nucleoid, thanks to nucleoid associated proteins (NAPs). The role of bacterial amyloid has recently emerged among these NAPs, particularly with the nucleoid-associated protein Hfq that plays a direct role in DNA compaction. In this chapter, we present a 3D imaging technique, cryo-soft X-ray tomography (cryo-SXT) to obtain a detailed 3D visualization of subcellular bacterial structures, especially the nucleoid. Cryo-SXT imaging of native unlabeled cells enables observation of the nucleoid in 3D with a high resolution, allowing to evidence in vivo the role of amyloids on DNA compaction. The precise experimental methods to obtain 3D tomograms will be presented.

KW - Bacterial amyloid

KW - Cryo-tomography

KW - Nucleoid

KW - X-ray imaging

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SN - 9781071625316

T3 - Methods in Molecular Biology

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BT - Bacterial Amyloids

A2 - Arluison, Véronique

A2 - Wien, Frank

A2 - Marcoleta, Andrés

PB - Humana Press

CY - New York NY USA

ER -

Cossa A, Wien F, Turbant F, Kaczorowski T, Węgrzyn G, Arluison V et al. Evaluation of the Role of Bacterial Amyloid on Nucleoid Structure Using Cryo-Soft X-Ray Tomography. In Arluison V, Wien F, Marcoleta A, editors, Bacterial Amyloids: Methods and Protocols. 1st ed. New York NY USA: Humana Press. 2022. p. 319-333. (Methods in Molecular Biology). doi: 10.1007/978-1-0716-2529-3_21

Evaluation of the Role of Bacterial Amyloid on Nucleoid Structure Using Cryo-Soft X-Ray Tomography

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