Evaluation of the antimicrobial activity of the mastoparan Polybia-MPII isolated from venom of the social wasp Pseudopolybia vespiceps testacea (Vespidae, Hymenoptera)

Juliana C. Silva, Lázaro M. Neto, Rogério C. Neves, Jaqueline C. Gonçalves, Monalisa M. Trentini, Ricardo Mucury-Filho, Karina S. Smidt, Isabel C. Fensterseifer, Osmar N. Silva, Lilian D. Lima, Patricia B. Clissa, Nathália Vilela, Fernanda Guilhelmelli, Luciano P. Silva, Marisa Rangel, André Kipnis, Ildinete Silva-Pereira, Octavio L. Franco, Ana P. Junqueira-Kipnis, Anamelia L. BoccaMárcia R. Mortari

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15 Citations (Scopus)

Abstract

Mastoparans, a class of peptides found in wasp venom, have significant effects following a sting as well as useful applications in clinical practice. Among these is their potential use in the control of micro-organisms that cause infectious diseases with a significant impact on society. Thus, the present study describes the isolation and identification of a mastoparan peptide from the venom of the social wasp Pseudopolybia vespiceps and evaluated its antimicrobial profile against bacteria (Staphylococcus aureus and Mycobacterium abscessus subsp. massiliense), fungi (Candida albicans and Cryptococcus neoformans) and in vivo S. aureus infection. The membrane pore-forming ability was also assessed. The mastoparan reduced in vitro and ex vivo mycobacterial growth by 80% at 12.5 µM in infected peritoneal macrophages but did not affect the shape of bacterial cells at the dose tested (6.25 µM). The peptide also showed potent action against S. aureus in vitro (EC50 and EC90 values of 1.83 µM and 2.90 µM, respectively) and reduced the in vivo bacterial load after 6 days of topical treatment (5 mg/kg). Antifungal activity was significant, with EC50 and EC90 values of 12.9 µM and 15.3 µM, respectively, for C. albicans, and 11 µM and 22.70 µM, respectively, for C. neoformans. Peptides are currently attracting interest for their potential in the design of antimicrobial drugs, particularly due to the difficulty of micro-organisms in developing resistance to them. In this respect, Polybia-MPII proved to be highly effective, with a lower haemolysis rate compared with peptides of the same family.

Original languageEnglish
Pages (from-to)167-175
Number of pages9
JournalInternational Journal of Antimicrobial Agents
Volume49
Issue number2
DOIs
Publication statusPublished - 1 Feb 2017
Externally publishedYes

Keywords

  • Candida albicans
  • Cryptococcus neoformans
  • Mycobacterium abscessus subsp. massiliense
  • Peptide
  • Staphylococcus aureus
  • Wasp venom

Cite this

Silva, J. C., Neto, L. M., Neves, R. C., Gonçalves, J. C., Trentini, M. M., Mucury-Filho, R., Smidt, K. S., Fensterseifer, I. C., Silva, O. N., Lima, L. D., Clissa, P. B., Vilela, N., Guilhelmelli, F., Silva, L. P., Rangel, M., Kipnis, A., Silva-Pereira, I., Franco, O. L., Junqueira-Kipnis, A. P., ... Mortari, M. R. (2017). Evaluation of the antimicrobial activity of the mastoparan Polybia-MPII isolated from venom of the social wasp Pseudopolybia vespiceps testacea (Vespidae, Hymenoptera). International Journal of Antimicrobial Agents, 49(2), 167-175. https://doi.org/10.1016/j.ijantimicag.2016.11.013