Polymerase chain reaction (PCR) is the basis of recombinant and other molecular biological techniques. Availability of cheap and robust PCR platforms enables the tests to be performed easily, even in resource constrained settings. Herein we compared the efficacy of a portable thermal cycler (Palm PCR™ G1-12 System) for rapid DNA amplification against the standard Peltier-based thermal cycler using plasmid DNA and genomic DNA in single and multiplex PCR experiments. Our study revealed that the Palm PCR™ G1-12 System could be a portable DNA amplification system to conduct various molecular techniques, especially in places where resources are limited.
|Number of pages||6|
|Journal||Songklanakarin Journal of Science and Technology|
|Publication status||Published - Jul 2016|
- Convective PCR
- Hand-held PCR
- Portable thermal cycler