Evaluation of microrna expression in patient bone marrow aspirate slides

Leah Morenos, Richard Saffery, Francoise Mechinaud, David Ashley, Ngaire J Elwood, Jeffrey Mark Craig, Nicholas C. Wong

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Like formalin fixed paraffin embedded (FFPE) tissues, archived bone marrow aspirate slides are an abundant and untapped resource of biospecimens that could enable retrospective molecular studies of disease. Historically, RNA obtained from slides is limited in utility because of their low quality and highly fragmented nature. MicroRNAs are small (≈22 nt) non-coding RNA that regulate gene expression, and are speculated to preserve well in FFPE tissue. Here we investigate the use of archived bone marrow aspirate slides for miRNA expression analysis in paediatric leukaemia. After determining the optimal method of miRNA extraction, we used TaqMan qRT-PCR to identify reference miRNA for normalisation of other miRNA species. We found hsa-miR-16 and hsa-miR-26b to be the most stably expressed between lymphoblastoid cell lines, primary bone marrow aspirates and archived samples. We found the average fold change in expression of hsa-miR-26b and two miRNA reportedly dysregulated in leukaemia (hsa-miR-128a, hsa-miR-223) was <0.5 between matching archived slide and bone marrow aspirates. Differential expression of hsa-miR-128a and hsa-miR-223 was observed between leukaemic and non-leukaemic bone marrow from archived slides or flash frozen bone marrow. The demonstration that archived bone marrow aspirate slides can be utilized for miRNA expression studies offers tremendous potential for future investigations into the role miRNA play in the development and long term outcome of hematologic, as well as non-hematologic, diseases.

Original languageEnglish
Article numbere42951
JournalPLoS ONE
Volume7
Issue number8
DOIs
Publication statusPublished - 13 Aug 2012
Externally publishedYes

Cite this

Morenos, L., Saffery, R., Mechinaud, F., Ashley, D., Elwood, N. J., Craig, J. M., & Wong, N. C. (2012). Evaluation of microrna expression in patient bone marrow aspirate slides. PLoS ONE, 7(8), [e42951]. https://doi.org/10.1371/journal.pone.0042951
Morenos, Leah ; Saffery, Richard ; Mechinaud, Francoise ; Ashley, David ; Elwood, Ngaire J ; Craig, Jeffrey Mark ; Wong, Nicholas C. / Evaluation of microrna expression in patient bone marrow aspirate slides. In: PLoS ONE. 2012 ; Vol. 7, No. 8.
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Morenos, L, Saffery, R, Mechinaud, F, Ashley, D, Elwood, NJ, Craig, JM & Wong, NC 2012, 'Evaluation of microrna expression in patient bone marrow aspirate slides' PLoS ONE, vol. 7, no. 8, e42951. https://doi.org/10.1371/journal.pone.0042951

Evaluation of microrna expression in patient bone marrow aspirate slides. / Morenos, Leah; Saffery, Richard; Mechinaud, Francoise; Ashley, David; Elwood, Ngaire J; Craig, Jeffrey Mark; Wong, Nicholas C.

In: PLoS ONE, Vol. 7, No. 8, e42951, 13.08.2012.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Saffery, Richard

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AU - Ashley, David

AU - Elwood, Ngaire J

AU - Craig, Jeffrey Mark

AU - Wong, Nicholas C.

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Morenos L, Saffery R, Mechinaud F, Ashley D, Elwood NJ, Craig JM et al. Evaluation of microrna expression in patient bone marrow aspirate slides. PLoS ONE. 2012 Aug 13;7(8). e42951. https://doi.org/10.1371/journal.pone.0042951