Evaluation of bioactive potential of an aloe vera sterol extract

Raksha Bawankar, V. C. Deepti, Pooja Singh, Rathinasamy Subashkumar, Govindasamy Vivekanandhan, Subramanian Babu

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23 Citations (Scopus)

Abstract

We prepared a crude gel material from Aloe vera succulent leaf tissues. The ethanolic extract of lyophilized A. vera gel was used for the GC-MS analysis. Hexadecanoic acid (22.22%) was identified as major compound. Sitosterol and stigmasterol were found to be 2.89% and 2.1% in the extract. HPLC analysis was carried out to confirm the presence of stigmasterol. The concentration of sterol extract needed to scavenge DPPH free radical by 50% was calculated as 5.2 mg mL-1. In the FRAP assay, the sterol extract showed significant hydroxyl radical scavenging in a dose-dependent manner (IC50 value 1.17 μg mL-1). Concentration of the sample required to reduce lipid peroxidation was found to be 4.18 μg mL-1, and the extract also possessed acetylcholinesterase activity (IC50 - 5.26 μg mL-1). Catalase activity was 0.196 μM H2O2 decomposed min-1 μg-1 protein, whereas the peroxidase activity was 17.01 μM of pyragallol oxidized min-1 μg -1 protein. The extract recorded higher activity against growth of S. greseus and C. albicans in the experiments carried out to determine antibacterial and antifungal activity, respectively.

Original languageEnglish
Pages (from-to)864-868
Number of pages5
JournalPhytotherapy Research
Volume27
Issue number6
DOIs
Publication statusPublished - Jun 2013
Externally publishedYes

Keywords

  • Aloe vera
  • antimicrobial
  • antioxidant
  • sterol

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