Evaluation of a novel Vi conjugate vaccine in a murine model of salmonellosis

Christine Hale, Frances Bowe, Derek Pickard, Simon Clare, Jean Francois Haeuw, Ultan Powers, Nathalie Menager, Pietro Mastroeni, Gordon Dougan

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15 Citations (Scopus)

Abstract

Immunisation of BALB/c mice with a vaccine containing Vi polysaccharide conjugated to the Klebsiella pneumoniae outer membrane 40 kDa protein (rP40), in combination with Escherichia coli heat-labile toxin adjuvant (LT), elicited anti-Vi IgG antibodies after administration using different routes. Testing of the immune serum in opsonisation assays demonstrated the specific enhancement of Vi-positive bacterial uptake by cultured murine bone marrow derived macrophages. Intra-peritoneal challenge of mice immunised with the Vi-based vaccine elicited a degree of protection against virulent Vi+ Salmonella enterica serovar typhimurium (S. typhimurium). In contrast, Vi vaccination did not confer protection against oral challenge with virulent Vi-positive S. typhimurium or S. dublin.

Original languageEnglish
Pages (from-to)4312-4320
Number of pages9
JournalVaccine
Volume24
Issue number20
DOIs
Publication statusPublished - 15 May 2006
Externally publishedYes

Keywords

  • Antibody
  • Salmonella
  • Typhoid
  • Vaccination
  • Vi polysaccharides

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