TY - JOUR
T1 - Evaluation of a new multi-parallel line immunoassay for systemic sclerosis-associated antibodies in an Asian population
AU - Low, Andrea H L
AU - Wong, Stephen
AU - Thumboo, Julian
AU - Ng, Swee Cheng
AU - Lim, Jia Yuen
AU - Ng, Xinyi
AU - Earnest, Arul
AU - Fong, Kok Yong
PY - 2012/8
Y1 - 2012/8
N2 - Objectives. To compare the SSc-line immunoassay (LIA) with conventional techniques of antibody detection, to evaluate its diagnostic utility and to describe clinical associations of antibodies in Asian SSc patients. Methods. Stored sera from patients with SSc (n = 68), SLE (n = 49), OA (n = 41) and normal controls (NCs, n = 32) were evaluated. Cohen's κ and Bland-Altman plots were used to evaluate agreement. Results. There was good agreement between LIA and ELISA for anti-Scl-70 (κ = 0.97), anti-CENPA (κ = 0.83), anti-CENPB (κ = 0.96) and anti-PmScl100 (κ = 1.00) (5.48-8.22% of values outside the 95% limits of agreement using Bland-Altman plots), and between LIA and IIF for anti-CENPA (κ = 0.81) and anti-CENPB (κ = 0.77) (P<0.001). Using LIA, of 32 (32/68, 47%) SSc patients negative for anti-Scl-70 and anti-CENPA/B, 5 (5/32, 15%) were positive for anti-Ku, -Nor90, -fibrillarin and -RP155. Specificity of each antibody for SSc was at least 97% (vs OA/NC) and 94% (vs SLE), except for anti-Ro52 (63%). Anti-CENPB was associated with joint pain [odds ratio (OR) 0.17], interstitial lung disease (OR 0.24) and telangiectasia (OR 4.00) (P<0.05). Anti-Ro60 was associated with pulmonary arterial hypertension (OR 3.89, P = 0.041). Conclusion. The SSc-LIA has good agreement with conventional techniques for selected antibodies and has good diagnostic utility.
AB - Objectives. To compare the SSc-line immunoassay (LIA) with conventional techniques of antibody detection, to evaluate its diagnostic utility and to describe clinical associations of antibodies in Asian SSc patients. Methods. Stored sera from patients with SSc (n = 68), SLE (n = 49), OA (n = 41) and normal controls (NCs, n = 32) were evaluated. Cohen's κ and Bland-Altman plots were used to evaluate agreement. Results. There was good agreement between LIA and ELISA for anti-Scl-70 (κ = 0.97), anti-CENPA (κ = 0.83), anti-CENPB (κ = 0.96) and anti-PmScl100 (κ = 1.00) (5.48-8.22% of values outside the 95% limits of agreement using Bland-Altman plots), and between LIA and IIF for anti-CENPA (κ = 0.81) and anti-CENPB (κ = 0.77) (P<0.001). Using LIA, of 32 (32/68, 47%) SSc patients negative for anti-Scl-70 and anti-CENPA/B, 5 (5/32, 15%) were positive for anti-Ku, -Nor90, -fibrillarin and -RP155. Specificity of each antibody for SSc was at least 97% (vs OA/NC) and 94% (vs SLE), except for anti-Ro52 (63%). Anti-CENPB was associated with joint pain [odds ratio (OR) 0.17], interstitial lung disease (OR 0.24) and telangiectasia (OR 4.00) (P<0.05). Anti-Ro60 was associated with pulmonary arterial hypertension (OR 3.89, P = 0.041). Conclusion. The SSc-LIA has good agreement with conventional techniques for selected antibodies and has good diagnostic utility.
KW - Autoantigens and autoantibodies
KW - Biomarkers
KW - Immunological techniques
KW - Laboratory diagnosis
KW - Scleroderma and related disorders
UR - https://www.scopus.com/pages/publications/84865154869
U2 - 10.1093/rheumatology/kes055
DO - 10.1093/rheumatology/kes055
M3 - Article
C2 - 22491717
AN - SCOPUS:84865154869
SN - 1462-0324
VL - 51
SP - 1465
EP - 1470
JO - Rheumatology
JF - Rheumatology
IS - 8
ER -